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DNA 的提取与含量测定. Dr.Chen Gao cg_npwr@yahoo.com.cn. DNA , DNP. 变性蛋白,肝糜. 氯仿. 100 ℃. 蓝色物. DNA +. 冰醋酸,少量硫酸. ( 二 ) 二苯胺法测定 DNA 含量. 二、原理 核酸含量的测定方法包括紫外吸收法、定磷法和分别针对 DNA 和 RNA 的颜色反应方法 本实验采用针对 DNA 的二苯胺法测 DNA 的含量。在酸性溶液中, DNA 与二苯胺共热生成蓝色化合物,该物质在 595nm 有最大吸收,在 40 ~ 400mg/mL 范围内其峰值与 DNA 含量成正比.
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DNA的提取与含量测定 Dr.Chen Gao cg_npwr@yahoo.com.cn CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
DNA,DNP 变性蛋白,肝糜 氯仿 CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka
100℃ 蓝色物 DNA + 冰醋酸,少量硫酸 (二)二苯胺法测定DNA含量 二、原理 核酸含量的测定方法包括紫外吸收法、定磷法和分别针对DNA和RNA的颜色反应方法 本实验采用针对DNA的二苯胺法测DNA的含量。在酸性溶液中,DNA与二苯胺共热生成蓝色化合物,该物质在595nm有最大吸收,在40~400mg/mL范围内其峰值与DNA含量成正比 CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka