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E. Coli Testing. An Overview M. A. “Peachie” Cline Burlin, D.V.M. Regulatory Requirements. Written plan Collect and analyze samples Records. Species Tested - Meat. Cattle Swine Sheep Goats Horses Mules Other Equines. Species Tested - Poultry. Chickens Turkeys Ducks Geese
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E. Coli Testing An Overview M. A. “Peachie” Cline Burlin, D.V.M.
RegulatoryRequirements • Written plan • Collect and analyze samples • Records
Species Tested - Meat • Cattle • Swine • Sheep • Goats • Horses • Mules • Other Equines
Species Tested - Poultry • Chickens • Turkeys • Ducks • Geese • Guineas
Who Tests? • PLANT PERSONNEL IN SLAUGHTER PLANTS
How? • According to written plan • FSIS guidelines • FSIS video • AOAC test
Record Keeping • m/M values • Statistical process control • 13 test moving window • Maintain 12 months
FSIS Responsibilities • Basic Checklist • Other Checklist • Enforcement
Basic Checklist • When? • Special team 1/97 • Grant of inspection Basic Checklist 1. Written plan 2. Sampling 3. Records
Other Checklist • PBIS schedules Other Checklist 1. Correct species 2. Location 3. Frequency 4. Random selection 5. AOAC Method 6. Last 13 tests 7. CFU/ml or square cm 8. 12 months of records 9. m/M value 10. SPC
Enforcement • Withhold inspection • Basic noncompliance • Repetitive other noncompliance • Shipping contaminated product • Noncompliance Record • Individual other noncompliance