1 / 2

人參及人參皂苷 ;Rg1 與 N- 乙醯半胱胺酸在順氯氨鉑引發的腎毒性於純系小鼠的藥效評估

人參及人參皂苷 ;Rg1 與 N- 乙醯半胱胺酸在順氯氨鉑引發的腎毒性於純系小鼠的藥效評估.

catriona-kissane
Télécharger la présentation

人參及人參皂苷 ;Rg1 與 N- 乙醯半胱胺酸在順氯氨鉑引發的腎毒性於純系小鼠的藥效評估

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 人參及人參皂苷;Rg1與N-乙醯半胱胺酸在順氯氨鉑引發的腎毒性於純系小鼠的藥效評估人參及人參皂苷;Rg1與N-乙醯半胱胺酸在順氯氨鉑引發的腎毒性於純系小鼠的藥效評估 • 順氯氨鉑（cisplatin， CDDP）是臨床上治療固體癌的常用化學治療藥物，其所引起的腎毒性常是限制臨床使用的主要原因。本研究的目的即在於評估人參及其純成分人參皂苷與N-乙醯半胱胺酸（N-acetylcysteine）作為預防藥物於CDDP所引起的腎炎之預防效果。實驗以6週齡雌鼠(BALB/c mice, female)，經腹腔連續五天給予CDDP 5 mg/kg/d以引發CDDP腎炎。在給予CDDP前五天開始經口單獨投予小鼠人參濃縮劑(ginseng extract，GE) 250 mg/kg/d或人參皂苷(ginsengoside，GS) Rg1 5 mg/kg/d及並分別合併N-acetylcysteine 450 mg/kg/d作為預防藥物。實驗結果顯示，給予GE、GS Rg1及N-acetylcysteine對於N-acetyl-beta-D-glucosaminidase (NAG) 、尿中肌酸酐（urine creatinine）、尿蛋白（urine protein）與血中尿素氮（BUN）皆有不同程度的改善效果；腎組織損傷相較於對照組也有減緩的趨勢。在免疫螢光染色方面，TNF-alpha(tumor necrosis factor-alpha)的量明顯受到抑制，p21及PCNA（proliferating cell nuclear antigen）的表現亦有不同程度的增加。綜合實驗結果，合併治療組對於預防CDDP所引起的腎毒性效果最佳。因此可以推論，經口投予人參濃縮劑、人參皂苷Rg1、N-acetylcysteine可以藉由抑制發炎反應、阻止細胞週期的前進並促進DNA修復以達到腎臟保護的效果。

  2. Effects of ginseng , ginsenoside Rg1 and N-acetylcysteine on cisplatin-induced nephrotoxicity in inbred mice • Cisplatin (CDDP) is one of the most commonly used antineoplastic agents for the solid tumor treatment. The major side effect of CDDP is nephrotoxicity. It is dose-related and has become a chief limitation of its clinical use. The purpose of this study was to evaluate the preventive effects of ginseng extract (GE), its active component, ginsenoside Rg1(GS Rg1) and N-acetylcysteine (NAC) on CDDP-induced nephrotoxicity in bred mice. Six-week-old female BALB/c mice were administered with 5 mg/kg of CDDP intraperitoneally once daily for 5 days. 250 mg/kg of GE or 5 mg/kg of GS Rg1 combination with 450 mg/kg/d of NAC were given orally once a day from 5 days before CDDP administration. Urinary N-acetyl-??-D-glucosaminidase (NAG), urinary creatinine(Ucr) and blood urea nitrogen (BUN) were determined, Renal tissues were served to histological examination. The antibodies including tumor necrosis factor-alpha(TNF-alpha), p21 and proliferating cell nuclear antigen (PCNA) was chosen to recognize the specific antigens that deposited in injury sites. Our findings demonstrated that GE, GS Rg1 and NAC attenuate CDDP-induced nephrotixicity by inhibiting TNF-alpha expression and inducing cell cycle arrest to repair DNA damage.According to this study, the effect of combination treatment was superior to other group.

More Related