Assessment of Luciferase Reporter Activity in Raw264.7 Cells with C/EBP Beta 3' UTR

DESCRIPTION

This supplementary figure presents the luciferase reporter activity assessment in Raw264.7 cells transfected with psiCHECK2 constructs. One construct bears the C/EBP beta 3’ UTR, while the other contains a mutant version with a disrupted miR-155 target site. Cells were treated with 100 ng/ml LPS for 18 hours. Results are expressed as mean ± SD from triplicate experiments, with statistical significance denoted (***) for p < 0.001. The data aids in understanding the regulatory role of miR-155 in the context of inflammatory responses.

Download

1 / 1

Feb 07, 2026

Télécharger la présentation

Assessment of Luciferase Reporter Activity in Raw264.7 Cells with C/EBP Beta 3' UTR

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

Raw264.7 *** Supplementary Figure 1. Assessment of luciferase reporter activity in Raw264.7 cells transfected with either the Renilla/firefly luciferase psiCHECK2 construct harbouring the c/ebp Beta 3´ UTR, or the psiCHECK2 construct c/ebp Beta 3’ UTR Mut with a mutated miR-155 target site (position 554-561; AGCAUUAA) before treatment with 100 ng/ml LPS for 18h. Values represent mean ±SD (n=3) (*** indicates p< 0.001). Figure 1 Supplementary (Worm, Stenvang et al., 2009)

More Related