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Lentikats Biotechnology in distilleries

Lentikats Biotechnology in distilleries. Content. 1. PRINCIPLES Principles of Lentikats Biotechnology Benefits of Lentikats Biotechnology Lentikats Biotechnology in distilleries 2. CASE STUDIES Case study – molasses distillery Case study – distillery processing sugar solutions

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Lentikats Biotechnology in distilleries

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  1. Lentikats Biotechnologyin distilleries

  2. Content • 1. PRINCIPLES • Principles of Lentikats Biotechnology • Benefits of Lentikats Biotechnology • Lentikats Biotechnology in distilleries • 2. CASE STUDIES • Case study – molasses distillery • Case study – distillery processing sugar solutions • Case study – corn distillery • 3. WHAT CAN WE OFFER? • References and experiences, our partners • Contacts

  3. Principles of Lentikats Biotechnology

  4. What is a Lentikats Biocatalyst? Unique shape of the matrix and the original principle of immobilisation enable the encapsulation of free enzymes or microorganisms (bacteria, yeasts, fungi) in a stable porous PVA (polyvinylalcohol) carrier. The carrier‘s advantages are: Excellent physio-mechanical characteristics that provide for long-term mechanical stability Low biodegradability Zero toxicity Lentikats Biocatalyst enables easy separation of enzymes and microorganisms from the surrounding media and therefore it can be reused multiple times. Biotechnologies are changing the world…

  5. Lentikats Biocatalyst Advantages of using PVA as a matrix for encapsulation: inexpensive, non-toxic polymerization under optimal conditions, cell conservation enables creation of hydrogels with excellent mechanical properties no side effects in processes large capacity for cell building easily supervised processes stable processes easy separation from bulk media

  6. Lentikats Lentikats Biocatalyst& Biotechnology (ourproduct) (applicationprocess) Lentikats Biocatalyst Production Lentikats Biocatalyst Application

  7. How does Lentikats Biocatalyst function? Due to its maximum surface and at the same time minimum volume of the Lentikats Biocatalyst, the diffusion limitations associated with the transport of the substrate into the lens and the product out of the lens are significantly reduced.

  8. Production of Lentikats Biocatalyst

  9. Lentikats Biocatalyst Production plant in Stráž pod Ralskem

  10. Benefits of Lentikats Biotechnology

  11. What are the advantages of Lentikats Biotechnology over traditional technologies? 1) Higher biomass concentration Reduced volume of reactors Reaction rate Investment costs saving Biomass concentration 100 € 100 € 100 € 100 € Operational costs saving (electricity, heat, etc.) Higher yields

  12. What are the advantages of Lentikats Biotechnology over traditional technologies? 100 € 100 € 2) Easy separation from bulk media 100 € Operationalcosts saving 100 € Repeatable use of Biocatalyst Lower production of waste and by-products 3) Robustness towards environmental shocks profound change of pH level higher concentration of toxic compounds Applicability under extreme or stress conditions

  13. Lentikats Biotechnology in distilleries

  14. Technologicalprocess set-up Legend: Sugars media inlet Fermented media Inlet for Lentikats Biocatalyst filling Outlet for Lentikats Biocatalyst emptying Sieve separator (angle slop 15-20°, aperture 0.8-1 mm) Liquid fermentation broth with Lentikats Biocatalyst Liquid fermentation broth without Lentikats Biocatalyst Gear with speed change box Stirrer pH probe well Temperature probe well Antifoam agent inlet Liquid level measurement (stainless steel pressure probe)

  15. Which substrates canbeusedforLentikats Biotechnology? Lentikats Biocatalysts provide a solution for the production of bioethanol from the following substrates: • Molasses, beet juice and sugar cane • Starch-containing (cereal) materials (corn, wheat, etc.) • Ligno-cellulose containing materials (straw, pollard…)

  16. Our products • Lentikats Biocatalyst for thefermentation of sucrose, glucose and lactose solutions (immobilized bacteria Zymomonas mobilis, yeast Saccharomyces cerevisiae, Kluyveromyces sp.) • Lentikats Biocatalyst for the2nd step of hydrolyses of liquefied starch solution (immobilized enzyme glukoamylase) • Lentikats Biocatalyst forthe production of inverted sugar from molasses (immobilized enzyme invertase) Energy is all around us……the key is to obtain it effectively… …and protect the environment at the same time…

  17. β

  18. Case study – molasses distillery

  19. Molasses distillery (125,000 L A.A. per day, continuous mode) 45 tons of Lentikats Biocatalyst (TFS* 48 %) 41 667  m3/year a.a. 404 tons/day 130 g/L TFS * Total Fermentable Sugars

  20. Fermentation conditions • Saccharomyces cerevisiae • continuous mode • 180 g sucrose . hour-1 . kg-1 • ethanolyield 95 % • 8.4 vol. % ethanol

  21. Economic comparison of traditional technology and Lentikats Biotechnology for molasses distillery Biotechnologiesare changing the world…..

  22. Reactor volume reduction– reduced investments Biotechnologiesare changing the world…..

  23. Lentikats Biocatalyst consumption (125,000 L a.a. per day) Biotechnologiesare changing the world….. * Guaranteed lifetime** Assumed lifetime Biotechnologies that are environmentally friendly …

  24. Profit of ethanol production at the capacityof125,000 La.a. per day Biotechnologiesare changing the world….. Solution for higher efficiency and stability…

  25. Case study – distillery processing sugar solutions (sugar cane or sugar beet juice)

  26. Sugardistillery(125,000 L a.a. perday, continuousmode) 45 tons of Lentikats Biocatalyst sugar solution 41 667  m3/year a.a. 140 g/L TFS * * Total Fermentable Sugars

  27. Fermentation conditions Biotechnologiesare changing the world….. • Zymomonas mobilis • continuous mode • 180g saccharose. hour-1 . kg-1 • temperature: 30–35°C,pH 4.8–5 • ethanol yield 95 % • 9.02 vol. % ethanol Biotechnologiesare changing the world…..

  28. Economic comparison of traditional technology and Lentikats Biotechnology for distillery processing sugar solutions

  29. Lentikats Biocatalyst consumption(125,000 L a.a. per day) Biotechnologiesare changing the world….. Biotechnologiesare changing the world….. * Guaranteed lifetime, ** assumed lifetime Biotechnologies that are environmentally friendly …

  30. Profit of ethanol production at thecapacity of125,000 La.a. per day Solution for higher efficiency and stability…

  31. Case study – corn distillery

  32. Corndistillery(15,000 L a.a. per day, continuousmode) 6 tons of LB 1.8 tons of LB (60 % of starch) 5,000,000 m3/year a.a. 39 tons/day

  33. Hydrolysis conditions • glucoamylase • continuous mode • 600 g glucose . hod-1 . kg-1 • temperature: 38–40°C, pH 4.5–5 • conversion rate 95 % • outlet concentration 150 g/L

  34. Fermentation conditions Biotechnologiesare changing the world….. • Zymomonas mobilis • continuous mode • 180g glucose. hour-1 . kg-1 • temperature: 30–35°C,pH 4.8–5 • ethanol yield 95 % • 9.21 vol. % ethanol Biotechnologiesare changing the world…..

  35. Economic comparison of traditional technology and Lentikats Biotechnology for corn distillery

  36. Reactor volume reduction– reduced investments

  37. Lentikats Biocatalyst consumption(125,000 L a.a. per day) * guaranteed lifetime, ** assumed lifetime Biotechnologies that are environmentally friendly …

  38. Profit of ethanol production at the capacity of15,000 La.a. per day Solution for higher efficiency and stability…

  39. Other Lentikats Biotechnology Applications Lentikats Biocatalysts and applied Lentikats Biotechnology also deliver additional value and benefits in the following sectors : waste water treatment – nitrogen removal pharmaceutics – production of robust biocatalysts food industry – whey processing, production of special sugars carbohydrates and syrups Biotechnologies are changing the world…

  40. What can we offer?

  41. Lentikats Biotechnologyservices • Monitoring of specified technological procedures and conditions. • Periodical monitoring of Lentikats Biocatalyst’s activity. • Supply and replacement of Lentikats Biocatalyst in such periods and volumes to sustain the required Lentikats Biotechnology parameters. • Disposal of the used Biocatalyst.

  42. What can we offer to you? • Have we raised your interest in testing Lentikats • Biotechnology inyour own project? • We are prepared to supply a sufficient amount of our Biocatalyst and provide full assistance in order to convince you of the obvious advantages our technology has when applied in your distillery. • For all companies introducing new technologies, the first applications and end results are the most important ones. That is why we offer excellent conditions to potential clients in an effort to eliminate any of their risks.

  43. You can test our biocatalyst easily by yourself with your substrate in your own plant or plant of your choice to see the results. • We offer to you our assistance and full support at: • - pilot model installation • - installation into an existing reactor • Load of Biocatalyst for testing is free of charge. Guarantee of your investments and the investment return

  44. References and experiences, our partners

  45. Our know-how, references andexperiences • Production of bioethanol from sugarbeet molasses in Bioferm Kolín distillery with a capacity of 6 litres of absolute alcohol per hour. • Processing of technical-technological documentation for the supply to an agricultural refinery with a production capacity of cca 3 000 m3of bioethanol per year, based on our concept of Bio Plant 2015. • Processing of technical documentation for the delivery of a distillery with a production capacity of cca 450 hl of bioethanol per day utilising corn as a raw material. • Production of bioethanol from starch milk of type B in Krnov distillery with a capacity of 12 litres of absolute alcohol per hour. • Process optimisation and processing of technical documentation for a reconstruction of the existing distillery in India which uses cane molasses as a substrate.

  46. Our partners in the field of industrial applications

  47. Our partners in the field of research and development

  48. Thank you for attention Company Headquarters: LentiKat´s a.s. Evropska 423/178 160 00 Prague 6 Czech republic Tel.: + 420 224 362 461 Fax: + 420 224 362 463 E-mail: info@lentikats.eu Production plant and laboratories LentiKat´s a.s. Pod Vinicí 83 471 27 Stráž pod Ralskem Czech republic Tel.: + 420 487 851 393 Fax: + 420 487 851 399 E-mail: info@lentikats.eu

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