Analysis of PPP1R1A and MLK1 Expression via Microarray and Real-Time PCR in Ad Infected Cells

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This study investigates the expression levels of PPP1R1A and MLK1 in various adenovirus-infected cell lines, including BRK, 3Y1, and others through microarray and real-time PCR techniques. The results indicate differential expression patterns of these genes when calibrated against GAPDH across different adenoviral strains (Ad5, Ad12). Using Western blotting, we validate the expression levels of key proteins involved in cellular signaling pathways. Our findings contribute to understanding the molecular interactions that may influence adenoviral pathogenesis and cellular responses.

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May 12, 2026

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Analysis of PPP1R1A and MLK1 Expression via Microarray and Real-Time PCR in Ad Infected Cells

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B A key microarray real-time PCR Western blot PPP1R1A MLK1 GAPDH GAPDH +Ad5 BRK 3Y1 3Y1 +Ad12 BRK +Ad12 +Ad5 +Ad5 E1 +Ad12 E1 +Ad5 E1 +Ad12 E1 +Ad5 E1A +Ad5 E1A +Ad12 E1A +Ad12 E1A 50.32±0.34 54.36±0.18 37.85±0.29 40.88±0.24 456.16±0.22 907±0.23 PPP1R1A:GAPDH (calibrated to untransformed BRK cell line) MLK1:GAPDH (calibrated to untransformed BRK cell-line) 3Y1 +Ad12 +Ad5 +Ad5 E1 +Ad12 E1 +Ad12 E1A +Ad5 E1A 3Y1 +Ad5 E1 +Ad5 +Ad12 +Ad12 E1 +Ad5 E1A +Ad12 E1A RelA C GAPDH +Ad5 BRK 3Y1 +Ad12 +Ad5 E1 +Ad5 E1A +Ad12 E1 +Ad12 E1A RelA:GAPDH (calibrated to untransformed BRK cell line) 3Y1 +Ad12 +Ad5 +Ad5 E1A +Ad5 E1 +Ad12 E1 +Ad12 E1A

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