1 / 1

Effect of pH and Substrate Concentration on Lipoxygenase Activity of Recombinant CaLOX1

This study investigates the lipoxygenase (LOX) activity of recombinant His-tag-CaLOX1 expressed in E. coli BL21 (DE3). We analyze the effects of pH on enzyme function using phosphate buffers and linoleic acid (0.1 mM) as the substrate. Additionally, time courses for CaLOX1 LOX activity are assessed with both linoleic acid and arachidonic acid as substrates at varying concentrations. Our findings elucidate the impact of environmental conditions on LOX activity, providing insights for future research on lipid metabolism.

cher
Télécharger la présentation

Effect of pH and Substrate Concentration on Lipoxygenase Activity of Recombinant CaLOX1

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. A B 200 140 Linoleic acid Linoleic acid Arachidonic acid 120 150 100 80 Lipoxygenase activity (nkat mg-1) Lipoxygenase activity (nkat mg-1) 100 60 40 50 20 0 0 4 5 6 7 8 9 10 20 30 pH Reaction Time (min) C 250 Linoleic acid 5 μM 200 15 μM 30 μM 60 μM 150 Lipoxygenase activity (nkat mg-1) 100 μM 150 μM 100 200 μM 50 0 5 10 15 20 25 30 Reaction time (min) Supplemental Figure S3. LOX activity of recombinant His-tag-CaLOX1 expressed in E. coli BL21 (DE3). A, Effect of pH on CaLOX1 LOX activity. Purified CaLOX1 was analyzed for enzyme activity in phosphate buffers with different pH values using 0.1 mM linoleic acid as a substrate. B, Time courses of CaLOX1 LOX activity. Purified CaLOX1 was incubated with 0.1 mM of linoleic acid or arachidonic acid as a substrate. C, Time courses of the LOX activity of purified CaLOX1, as determined with various concentrations of linoleic acid as a substrate.

More Related