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Impact of Collagen Coating and Serum Treatment on Senescence-associated Beta-galactosidase Activity in OB-6 Cells

This study analyzed Senescence-associated Beta-galactosidase (SABG) activity in OB-6 cells treated with different concentrations of collagen coating and serum from OVX or LTBB-OVX rats for 3 days. Results show variations in SABG activity based on the treatment conditions.

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Impact of Collagen Coating and Serum Treatment on Senescence-associated Beta-galactosidase Activity in OB-6 Cells

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  1. Supplemental Table 2. Senescence associated beta-galactosidase (SABG) activity in OB 6 cells Relative SABG activity Control OVX serum BB+OVX serum 0.05 ug/cm2 Collagen coating 1.67±0.012 1.83±0.011# 1.65±0.017 20 ug/cm2 Collagen coating 1.47±0.010* 1.69±0.024#, * 1.46±0.034* Senescence associated beta-galactosidase activity analyses in total proteins isolated from calvarial cells cultured in different concentration of collagen coated wells and treated with OVX or LTBB-OVX rat serum for 3d. # p<0.05, versus control, * p<0.05, 0.05 μg/cm2 versus 20 μg/cm2 collagen coating, by t-test. Data are expressed as mean ± SEM with triplicates.

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