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Real-time Monitoring technology of Mass spectrometry

Real-time Monitoring technology of Mass spectrometry. Speaker : Achong. Outline. IMS PTR-MS SIFT-MS Application of IMS IMS-TOF IMS-IMS-TOF Application of PTR-MS PIT-MS. Real-time Monitoring Mass spectrometry. Environmental science Biochemical and medical diagnosis

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Real-time Monitoring technology of Mass spectrometry

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  1. Real-time Monitoring technology of Mass spectrometry Speaker : Achong

  2. Outline • IMS • PTR-MS • SIFT-MS • Application of IMS IMS-TOF IMS-IMS-TOF • Application of PTR-MS PIT-MS

  3. Real-time Monitoring Mass spectrometry • Environmental science • Biochemical and medical diagnosis • Workplace safety • Security check

  4. EI source • Analysis of atmospheric air is especially challenging using electron ionization mass spectrometry, because the gas loading of the ion source by N2 and O2 (as well as water vapor) can be excessive. 70 ev

  5. Real-time Monitoring MS • Soft chemical ionization • Measurement in Real-Time • Without pre-concentration system

  6. I. IMS (ion mobility spectrometer) Ion mobility mass spectrometry (Eiceman,1991; Baumbach, 2001) AnalyticalChemistry, A390-A397, November 1, 2004

  7. Ion mobility (K) JournalofBimolecularTechnique, Vol.13, Issue 2, June,2002

  8. Ion Mobility-Mass Spectroscopy Witt & Bowers: JACS.2000, 122, 3458.

  9. Ion mobility mass spectrometry Advantages: • Multiform ion source could be applied. • Some isomer could be separated. • Real-time measurement Disadvantages: • Lower mass resolution • Lower sensitivity to allow accurate analysis of complex mixtures. • Standard is requisite

  10. II. PTR-MS (proton-transfer reaction) • Proton-transfer reaction-mass spectrometry (Lagg A, Taucher J, Hansel A, Lindinger W. 1994.) http://info.uibk.ac.at/c/c7/c722/umwelt/html/animation.html

  11. proton-transfer reaction • H3O+ + R → RH+ + H20 • [RH+] = [H3O+]o(1 - e-k[R]t) = [H30+]o[R]kt J. Mass Spectrom Ion Processes, 173 (1998) 191-241

  12. J. Mass Spectrom Ion Processes, 173 (1998) 191-241

  13. J. Mass Spectrom Ion Processes, 173 (1998) 191-241

  14. PTR mass spectrometry Advantages: • Soft ionization and low matrix effect • Real-time measurement • Low limit of detection (0.5 ppbv for 1-min measurements) Disadvantages: • A large number of VOCs could not be identified and quantified.

  15. III. SIFT-MS (selected ion flow tube) • Adams NG, Smith D. 1976. • Spanel P ,Smith D. 1995. http://www.syft.com/

  16. SIFT mass spectrometry Advantages: • application of multiple reagent ions (H3O+, NO+ and O2+) • quantification in real-time • discriminate isomers in Real-Time Disadvantages: • The kinetic parameters of the reagent ion and the analyte ion must be known. Selected ion flow tube mass spectrometry for no-line trace gas analysis David Smith and Patrik Spanel Reference : Mass Spectrometry Reviews, 2005, 24, 661– 700

  17. Application of IMS IMS/TOF IMS/IMS/TOF

  18. Ion Mobility/TOFMS Anal. Chem. 2003, 75,6202-6208

  19. Pulse sequence of IMS & TOF Anal. Chem. 2003, 75,6202-6208

  20. example spectrum Analytical Chemistry, 1998 Vol. 70, No. 11, June 1,

  21. Anal. Chem. 2003, 75,6202-6208

  22. collision-induced dissociation http://proteomics.med.ncku.edu.tw/proteomicsweb/Liao_94_1007.ppt

  23. IMS/IMS/TOF Anal. Chem. 2006, 78,4161-4174

  24. operational mode A Anal. Chem. 2006, 78,4161-4174

  25. operational mode B Anal. Chem. 2006, 78,4161-4174

  26. operational mode C Anal. Chem. 2006, 78,4161-4174

  27. Application of PTRMS PIT-MS

  28. PIT-MS http://cires.colorado.edu/~gouw/Instruments/PITMS/PITMS.html

  29. PIT-MS • proton-transfer ion trap-mass spectrometry J Am Soc Mass Spectrom 2005, 16, 1316–1324

  30. CID process on ion-trap MS http://www.chem.agilent.com/cag/Other/IonTrap_fig2.gif

  31. calibration measurement of the PIT-MS J Am Soc Mass Spectrom 2005, 16, 1316–1324

  32. single CID measurement of m/z 59 ambient air pure acetone pure propanal J Am Soc Mass Spectrom 2005, 16, 1316–1324

  33. CID of propanal and acetone m/z=59 m/z=41 m/z=31 J Am Soc Mass Spectrom 2005, 16, 1316–1324

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