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Impact of Cytokines on Threshold Cycles in qPCR Analysis at Varied Temperatures

This study investigates the influence of various cytokines, including IL-12p40, TGF-ß1, IL-18, and TNF-α, on the threshold cycles (Ct) in quantitative PCR (qPCR) assays. By examining the effects of these key cytokines at different temperatures, we aim to elucidate their roles in immune response modulation. The data reveal correlations between cytokine levels, temperature variations, and resultant Ct values, providing insights into optimizing PCR conditions for cytokine quantification. This research has implications for understanding immune responses in health and disease.

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Impact of Cytokines on Threshold Cycles in qPCR Analysis at Varied Temperatures

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  1. A. IL-12p40 TGF-ß1 IL-18 IFN- ß2-µ TNF- IL-15 IL-1ß IL-10 IL-13 MIF 150 bp 110 bp 70 bp B. IFN- 0.16 0.14 0.12 0.10 0.08 0.06 0.04 0.02 0.00 -0.02 IL-1ß IL-15 IL-12p40 IL-18 IL-10 Derivative TNF- MIF IL-13 ß2-µ TGF-ß1 Temperature (°C) 75 80 C. 8 40 6 30 4 20 Threshold Cycle (Ct) Rn 2 10 107 1010 109 108 106 105 104 103 0 0 0 10 20 30 40 101 102 103 105 106 107 108 109 1010 104 Cycle Starting Quantity

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