1 / 1

Impact of Modified Start Codons on Luciferase Activity in CaMV 35S Promoters

This study investigates how specific modifications in the start codon context influence luciferase activity driven by the CaMV 35S promoter. We analyze various positions (-4 to +4) around the start codon (ATG) to assess their effect on translational efficiency. Data are presented as relative luciferase activity percentages at different concentrations for both modified and unmodified sequences of the long and 1x versions of the CaMV 35S promoter. The findings provide insights into optimizing translational efficiency in plant biotechnology.

clive
Télécharger la présentation

Impact of Modified Start Codons on Luciferase Activity in CaMV 35S Promoters

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. modified start codon context: A A C C ATG G (position: -4 -3 -2 -1 +1+2+3 +4) MV [%] SD (n) 133 45 (3) 100 - (3) CaMV 35S(long) (including mod. sequence) CaMV 35S(long) (without mod. sequence) 0 50 100 150 200 relative luciferase activity (%) MV [%] SD (n) 152 27 (3) 100 - (3) CaMV 35S(1x) (including mod. sequence) CaMV 35S(1x) (without mod. sequence) 0 50 100 150 200 relative luciferase activity (%)

More Related