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Calcium-Induced Calcium Release (CICR) Mechanism: Regulation and Characteristics

Explore the detailed mechanisms, characteristics, and regulation of Calcium-Induced Calcium Release (CICR) in cellular function, including its Ca2+ dependence, MgCl2 blocking, ATP dependency, and regulatory roles of CaM, NCS-1, and more. Discover how the release of apoCaM from IP3R by Ca2+ activates CICR. Visual aids show Ca2+, Mg2+, and ATP dependencies, as well as calcium-binding proteins involved in the process.

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Calcium-Induced Calcium Release (CICR) Mechanism: Regulation and Characteristics

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  1. Observation: CICR in A7r5 • not IP3R or RyR • no Passive exchange • 2. Characteristics of CICR • Ca2+ dependence • blocked by MgCl2 • ATP dependent • 3. Regulation • CaM has no effect • Inhibition by CaM1234, NCS-1, NCS-1 E120Q, sCaBP1, lCaBP1 • model: release of apoCaM from the IP3R by Ca2+ activates CICR (experiments with peptides !)

  2. Fig. 1

  3. No passive exchange Fig. 2

  4. Fig. 3 IP3R en RyR inhibitoren

  5. Fig. 4 Ca2+ Dependence

  6. Fig 5 Mg2+ Dependence ATP dependence

  7. Fig. 6a Fig. 6b

  8. Calcium-binding proteins Calmodulin Calmodulin1 Calmodulin1234 Long CaBP1 Short CaBP1 NCS-1/Frequenin NCS-1/FrequeninE120Q

  9. Fig. 8

  10. 45 min Load Efflux Load Load + CaM-binding peptide Ca 2+

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