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Time-Dependent Analysis of NF-κB Signaling Pathways in Response to Stimulation

This study investigates the time-dependent activation of key signaling proteins in the NF-κB pathway following stimulation. Data were collected at various time points (0, 0.5, 10, 30, 60, and 120 minutes) post-stimulation, assessing the changes in the levels of p-IKKα/β, p-ERK, p-p38, and p-JNK1/2. The results highlight differences in protein activation, illustrating the dynamic nature of the NF-κB signaling response. This research contributes to understanding cellular mechanisms involved in inflammation and immune responses.

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Time-Dependent Analysis of NF-κB Signaling Pathways in Response to Stimulation

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  1. Time after stimulation (min) Time after stimulation (min) 0 60 0 5 10 30 120 60 0 5 10 30 120 60 5 10 30 120 (Kda) (Kda) 150 150 100 100 IKK 75 75 75 IKK 50 50 50 37 IB 37 37 Time after stimulation (min) Time after stimulation (min) 0 60 0 5 10 30 120 60 5 10 30 120 (Kda) (Kda) 150 150 100 100 p-IKK/  75 75 50 50 Actin 37 37 The full scan for Fig. 2B Fig S1 Lee JY, et al Time after stimulation (min) (A) (+) (-) 0 5 10 30 60 120 NF-B The full scan for Fig. 2A (B) Time after stimulation (min) (Kda) 150 100

  2. Fig S2 Lee JY, et al (C) Unstimulated control Toxin A + Toxin A alone c-Rel RelB p50 p52 p65 NF-B The full scan for Fig. 2C (A) Time after stimulation (min) Time after stimulation (min) Time after stimulation (min) 0 60 0 5 10 30 120 60 0 5 10 30 120 60 5 10 30 120 (Kda) (Kda) (Kda) 150 150 150 100 100 100 75 75 75 50 50 50 p-ERK ERK p-p38 37 37 37 Time after stimulation (min) Time after stimulation (min) Time after stimulation (min) 0 60 0 5 10 30 120 0 60 60 5 10 30 120 5 10 30 120 (Kda) (Kda) (Kda) 150 150 150 100 100 100 75 75 75 50 50 p-JNK1/2 JNK1/2 50 37 37 p38 37 Time after stimulation (min) 0 60 5 10 30 120 (Kda) 150 100 75 50 Actin 37 The full scan for Fig. 6A

  3. Wild-type NF-B KO Toxin A PD98059 SB203580 SP600125 - - - - + - - - + + - - + - + - + - - + Toxin A BMS - - + - + + - - + - + + 120 (Kda) (Kda) 100 p-IKK/  75 p-IKK/  70 45 50 37 30 (Kda) (Kda) 120 100 75 70 50 p-ERK 45 37 Actin 30 The full scan for Fig. 7A (Kda) 100 75 50 p-p38 37 (Kda) 100 75 50 p-JNK 37 (Kda) 100 75 50 Actin 37 The full scan for Fig. 7B Fig S3 Lee JY, et al (A) (B) NF-B KO Wild-type Toxin A BMS - - + - + + - - + - + + NF-B Positive control Negative control Positive control Negative control The full scan for Fig. 7B

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