1 / 21

WORKING IN A BIOLOGICAL SAFETY CABINET

WORKING IN A BIOLOGICAL SAFETY CABINET. BTO Biological Services Ltd. Risk Group Definitions. Risk Group 1 Low individual and community risk e.g., E.coli K12, Lactobacilli,

cybele
Télécharger la présentation

WORKING IN A BIOLOGICAL SAFETY CABINET

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. WORKING IN A BIOLOGICAL SAFETY CABINET BTO Biological Services Ltd.

  2. Risk Group Definitions • Risk Group 1Low individual and community riske.g., E.coli K12, Lactobacilli, • Risk Group 2Moderate individual risk, low community riske.g., Staphylococcal species, enterotoxigenic Escherichia coli, Shigella species, Salmonella species • Risk Group 3High individual risk, low community riskM. tuberculosis, C.burnetii, • Risk Group 4High individual risk, high community riske.g., Ebola virus

  3. Biological Agent • Biological agents include but are not limited to:Bacteria ParasitesFungi Viral vectorsViruses Recombinant productsRickettsiae PrionsCultured human or animal cellsand the potentially infectious agents they might contain

  4. Biohazard • A biohazard is a biological agent or a biological material that is potentially hazardous to a life form • The manipulation of a biological agent or biological material so that the capacity or potential to produce infection or disease in a living form is present

  5. Aerosols • DefinitionAn aerosol is a liquid or particulate suspended in a gaseous medium • Size range is from 0.01 microns – 100 microns • Health relevant fractions of aerosolsinhalable 0.01-100 micronsnasopharyngeal deposition 25-100 micronstracheobronchial deposition 5-25 micronsalveolar deposition 0.01-5 microns

  6. Route of Transmission & Entry • Inhalation • Ingestion • Inoculation • Direct or indirect contact

  7. Biological Safety Cabinets • Bad terminology Laminar flow cabinets Tissue culture cabinets Sterile benches • Good terminology Biological Safety cabinets Biosafety cabinets • In general, BSC’s are classified according to the method by which air volumes are recirculated or exhausted.

  8. Air Cleaning Device • The primary air cleaning device in biological safety cabinets is a HEPA filter (High Efficiency Particulate in Air) • HEPA filters do not provide protection against volatiles gases or vapours – only particulates

  9. Filter Efficiency • HEPAA minimum efficiency of capture is 99.97% for 0.3 micronsAny particulate bigger or smaller than 0.3 microns is captured more efficiently than 99.97%

  10. Biosafety cabinet review • When do you work in a Class II BSC? • When there is potential to create aerosols • When you need to protect yourself, the product and the environment

  11. Types of BSC’s • Class IProtects the environment and the user – does not protect the productIt is a partial containment device and is suitable for work involving low to moderate risk agents (containment levels 1, 2 and 3) User and environmental protection is made possible by constant unidirectional movement of air into the cabinet, away from the user through HEPA filtration before re-entry into the environment. The product is not protected

  12. Types of BSC’s • Class IIThe Class II biological safety cabinet has three key features:(i) A front access opening with carefully maintained inward airflow.(ii) HEPA-filtered, vertical, unidirectional airflow within the work area. (iii) HEPA-filtered exhaust air to the room or exhaust to a facility exhaust system.

  13. Subtypes of Class II BSC’s • Class IIA1 predominant cabinet protects the product user and environment • Class IIA2protects the product user and environment • Class IIB1protects the product, user and environment can be used with small amounts of volatiles and gases • Class IIB2 protects the product, user and environment

  14. Class IIA BSC’s

  15. Class IIA BSC’s

  16. Class IIA BSC’s • Air curtain between sash and work surface is critical to containment

  17. Working in a Class IIA BSC • Whenever there is potential for aerosol generation you must work in a BSCe.g., manipulating human or animal tissues or fluids; opening a closed centrifuge cupafter centrifugation, taking spent media off Tissue culture. • You need to employ good techniquee.g., employ good start up procedures; disinfect everything you need to work with into the BSC prior to commencing work; do not block the grilles; be seated at the right height; ensure that nothing you need to use is outside the cabinet --------

  18. Working in a Class IIA1 BSC • Place everything you need to work with inside the cabinet • Purge to establish airflow • Separate clean items from dirty items • Don’t pass dirty items over clean ones • Do not block the front intake grille • Work on plastic backed absorbent material – for quick clean ups

  19. Fire in BSC

  20. UV Radiation • UV germicidal lamps produce UV light at 253.7 nm wavelength (40 Wcm-2) - this has limited penetrating power • The intensity or destructive capability of UV decreases by the square of the distance from the lamp • Exposure time is always related to distance • The intensity of the lamp also decreases with time

  21. UV Radiation • The intensity of the lamp is drastically affected by the accumulation of dust and dirt on it • UV lights should never be on while someone is working in the cabinet • UV lights should not be left on while anyone is in the area and likely to be exposed to reflected UV radiation

More Related