Analysis of Transcriptional Responses in MCF7 and BT474 Cell Lines Under Varying Treatments
This study examines the transcriptional changes in MCF7-AROM1 and BT474-AROM3 cell lines under different hormonal and pharmacological conditions, including E2 absence and treatment with RAD001, Letrozole, and 4-OHT. The data presents fold changes in transcription relative to control conditions, highlighting the effects of androgen-mediated signaling on estrogen receptor (ER) and estrogen response element (ERE) activity in these breast cancer models. Results are quantitatively assessed to elucidate the mechanisms of resistance and sensitivity to hormonal therapies.
Analysis of Transcriptional Responses in MCF7 and BT474 Cell Lines Under Varying Treatments
E N D
Presentation Transcript
Fig 5 A MCF7-AROM-1 basal (No E2) ER/ERE MCF7-AROM1 And-mediated ER/ERE Fold change in transcription relative to And control Fold change in transcription relative to DCC control * + + - - - + + - - + - + - + + + - + - - + + + - + + And 10nM RAD001 2nM Let 100nM 4-OHTam 10nM B BT474-AROM3 basal (No E2) ER/ERE BT474-AROM3 And-mediated ER/ERE * Fold change in transcription relative to And control Fold change in transcription relative to DCC control * * * + + - - - + + - - + - + - + + + - + - - + + + - + And 10nM RAD001 2nM Let 100nM 4-OHTam 10nM C LTED E2- mediated ER/ERE LTED basal (No E2) ER/ERE * * Fold change in transcription relative to DCC control Fold change in transcription relative to E2 control E2 0.01nM RAD001 2nM 4-OHTam 10nM + - - + + - + - + + + + D LTED + - - - + + - - + - + - + + + - + - + + + + + + DCC RAD001 2nM E2 0.01nM 4-OHTam 10nM pER ser118 0.19 0.15 0.82 0.87 0.70 0.87 pER ser167 0.86 0.02 0.11 0.02 1.39 0.01 ER pS6S240/S244 0.96 0.00 1.07 0.00 1.45 0.00 Actin
