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Due TOMORROW: Signed Syllabus/Honor code Signed Lab Safety Contract

Learn how to properly use a microscope, identify its parts, adjust magnification, and create accurate biological drawings in this comprehensive guide for biology students.

danielhope
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Due TOMORROW: Signed Syllabus/Honor code Signed Lab Safety Contract

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  1. Due TOMORROW: • Signed Syllabus/Honor code • Signed Lab Safety Contract 2) Spiral Notebook (College ruled is best) • PUT textbook in the assigned cabinet when you bring it in over the next week • Make sure you write down the #!!!!

  2. Anatomy of a Microscope

  3. Objective SWU: the proper way to use a microscope SW: Identify the major parts and functions of a microscope

  4. Microscope Guidelines Microscopes are expensive instruments. They must be handled with care in order to avoid damage. • Keep the instrument in a box or under a cover when not in use. • Use only the supporting arm of the microscope to lift it. Next >

  5. Directions • Each table should have a microscope • Identify each part of the microscope on the real one and label on your handout as we go

  6. Label The Microscope Parts Eyepiece Coarse Focus Body Tube Fine Focus Revolving Nosepiece Objective Lenses Objective Lenses Stage Clips Body/Arm Stage Diaphragm/Light Adjust Inclination joint Light Base

  7. 1. Find the Lowest Power Objective Lens and position it in the middle Always start your microscope lab with the lowest power objective lens in place Use the “short” lens first!!!!! Next >

  8. 2. Use the coarse focus knob to raise the objective lens Raise the microscope by turning the coarse focus knob all the way up Make it as “tall” as it can be Next >

  9. 3. Place the Slide on the Stage Place the slide on the stage, so that the specimen is over the middle of the hole on the stage. Make sure that light is shining through it and then secure the slide in place with the stage clips. Next >

  10. 4. Lower the Objective Lens to focus Lower the objective lens using the coarse focus dial SLOWLY, until the specimen comes into focus You MAY NOT do this on when using high power! You will crush the slide and/or break the lens Try to keep both eyes open. This will help to prevent eye strain and headaches. Next >

  11. 5. Adjusting the Diaphragm (if needed) The diaphragm can be adjusted to let different amounts of light through the specimen. This is because specimens vary in thickness. Trial and error Next >

  12. 6. Minor Focusing Adjustments The fine focus dial alters the height of the stage more accurately than the coarse focus dial. You may need to make minor adjustments to the objective lens using the fine focus dial to get your specimen into sharp focus. Next >

  13. 7. Change Magnification (as necessary) If needed, change magnification, on objective lens at a time. Always make sure your specimen is in focus BEFORE changing magnification Next >

  14. Total Magnification Eyepiece x Objective lens= TOTAL Magnification If a ×10 objective lens is used, assuming the eye piece magnification is ×10, the total magnification of the image will be (×10) × (×10) = ×100. Next >

  15. 8. Biological Drawings • Your drawings are a record of what you have seen. Make sure your pictures are as accurate as possible • Always draw AS you are looking at the specimen • Label as needed Next >

  16. Using the Microscope • Always start with the low powered objective lens (short) • Raise coarse focus knob all the way up (tall) • Place slide on stage- clip • SLOWLY, turn coarse focus knob down until specimen is clear • Change magnification as necessary

  17. Using the Microscope THE GOLDEN RULE 6. When using High power, NEVER EVER touch the coarse (big) focus knob

  18. Steps for Cleanup • Center the low power objective (short lens) • Raise coarse focus knob all the way up (tall) 3. Remove slide 4. Lower coarse focus all the way down (short) 5. Unplug cord 6. Wrap cord around base 7. Put cover back on

  19. Microscope Care Review • Always carry with 2 hands • Only use lens paper for cleaning • Do not force knobs • Always store covered • Keep objects clear of desk and cords (put excess cord on table)

  20. Materials to Get out • Honor code SIGNED • Lab Safety Contract SIGNED • Spiral Notebook

  21. Objective SWU: the proper way to use a microscope In pairs, SW: practice using microscopes to prepare for their future microscope labs

  22. Microscope Lab • Move to a lab table (1 pair on each side) • Send someone to get a microscope (max 2 per lab table) • Follow microscope set-up and BEGIN!!!!

  23. What are you looking at? • Sordaria= fungus • Allium= Onion root tip • Oedogonim= green algae • Volvox= green algae • Rbizopusw.m.= bread mold • Human sperm smear= sperm • Zea Mays Stem = corn stem • Allium Root Tip = onion root tip • Epithelia= simple cuboidal kidney tubules • Spirogyra Vegetative = Green algae • Tilia Stem = type of tree stem • Plasmodium Falciparum= Protozoan Parasite that causes Malaria in humans • Diatom= algae • Lilium leaf= Lilly leaf • Fasciola Hepatica- Parasitic flatworm that infects the liver. Causes “liver rot” Fasciola Hepatica

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