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Input DNA template sequences, product size range, database, etc

Dimer examination by PriDimerCheck. Primer specificity evaluation by MFEprimer. Input DNA template sequences, product size range, database, etc. Utilize Primer3 to design 5 (default) candidate primers for each of the DNA template sequences. 1 0 1 0 1 … 0 1 0 1 1 1 0 1 0 1 ….

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Input DNA template sequences, product size range, database, etc

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  1. Dimer examination by PriDimerCheck Primer specificity evaluation by MFEprimer Input DNA template sequences, product size range, database, etc Utilize Primer3 to design 5 (default) candidate primers for each of the DNA template sequences 1 0 1 0 1 … 0 1 0 1 1 1 0 1 0 1 … Draw a matrix based on the dimer and specificity check results for any two primers Mobility prediction based on Hellings’ formula (1974) Choose the best 15 PSCs using the “Graph expanding model” based on the scoring matrix and MinBS Output the best 15 PSCs in user-friendly format

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