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Petr GALUSZKA

FROM CYTOKININ METABOLISM TO AGRICULTURE. Petr GALUSZKA Centre of the Region Haná for Biotechnological and Agricultural Research Palacký University in Olomouc. CK qualitative content analysis. Gajdošová et al., JXB 2011. CK qualitative analysis during maize development.

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Petr GALUSZKA

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  1. FROM CYTOKININ METABOLISM TO AGRICULTURE Petr GALUSZKA Centre of the Region Haná for Biotechnological and Agricultural Research Palacký University in Olomouc

  2. CK qualitative content analysis Gajdošová et al., JXB 2011

  3. CK qualitative analysis during maize development isopentenyladenine trans-zeatin cis-zeatin dihydrozeatin pmol/g of leaf fresh weight kernel germination 20th day 20th day root development shoot development

  4. CYTOKININ SENSING in Zea mays Histidine kinase – CK receptor bound to membranes Contrary to Arabidopsis receptors, there is higher sensitivity to cZ and DHZ at least on some maize receptors S. Lomin et al., JXB 2011

  5. zeatin metabolism in planta is rather obscure ? ? CYP450 tZ zeatin reductase DHZ zeatin isomerase iP proved in planta gene is known proved in vitro activity detected hypothesized IPT LOG cZ de novo biosynthesis tRNA degradation

  6. Cytokinin dehydrogenases have different preferences CKX relative activity CKX gene families: Arabidopsis thaliana 7 genes Zea mays 13 genes Oryza sativa 11 genes Hordeum vulgare11 genes

  7. CKX as a transgene Constitutive overexpression of CKX causes cytokinin-deficient phenotype Main function of CKs on plant differentiation proved together with opposite role on root and shoot development Cytokinin content 30–50% of WT Construct: 35S::HvCKX2

  8. Transformation of Barley with CKX genes Constitutive CKX overexpression in cereal: prematured senescence no onset of flowering (sterility) enlarged root system (dependent of transgene) Ubiqutin::ZmCKX1 Z10 WT Ubiqutin::HvCKX2 WT trsg WT Z2 Z4 Z7 Z8 Z10

  9. CKX and IPT gene families in barley 11 CKX genes2 tRNA IPT genes 5 de novo IPT genes

  10. wild type situation our transgenic approach

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