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Manipulating molecules

Manipulating molecules. Biot-111 Lecture 4. Types of molecules. Nucleic Acids Deoxyribonucleic acid (DNA) Chromosomal DNA Mitochondrial DNA (mtDNA, or less popularly, mDNA) Chloroplast DNA Ribonucleic acid (RNA) Ribosomal RNA (rRNA) Messenger RNA (mRNA) Transfer RNA (tRNA)

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Manipulating molecules

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  1. Manipulating molecules Biot-111 Lecture 4

  2. Types of molecules • Nucleic Acids • Deoxyribonucleic acid (DNA) • Chromosomal DNA • Mitochondrial DNA (mtDNA, or less popularly, mDNA) • Chloroplast DNA • Ribonucleic acid (RNA) • Ribosomal RNA (rRNA) • Messenger RNA (mRNA) • Transfer RNA (tRNA) • Complementary DNA (cDNA) RNA-DNA Hybrid

  3. Proteins • Structural proteins • E.g. Membrane proteins • Functional proteins • E.g. Enzymes • Helicases • Transferases • Proteases • Nucleases • Kinases • Phosphatases etc.

  4. Manipulating Nucleic Acids / cloning • Isolation and Purification of Nucleic Acid • Restriction analysis • Selection of suitable vector • Recombination • Insertion into a suitable host • Screening and selection of recombinants

  5. The tools of recombinant DNA technology

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