Construction of a Multicistronic Vector for EGFP Expression with Insulator Elements

DESCRIPTION

This project focuses on the design of a multicistronic vector incorporating essential elements for effective gene expression. The vector features restriction sites from HindIII, BamHI, EcoRI, and XhoI, along with an insulator element to enhance transcriptional regulation. The backbone includes a promoter for EGFP expression followed by a SV40 polyadenylation signal. Designed for optimal use in mammalian cells, the vector is being characterized for stability and expression efficiency. Key fragments of 347/478, 504/412, and 500bp are incorporated to facilitate robust gene expression.

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Feb 12, 2026

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Construction of a Multicistronic Vector for EGFP Expression with Insulator Elements

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HindIII HindIII HindIII BamHI EcoRI EcoRI XhoI AflII AflII AflII SphI NotI Insulator f1 origin UM promoter EGFP SV40 pA 347/478 347/478 504/412 500bp

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