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Genome-Wide Survey of Cellulase-Related Genes in Pleurotus ostreatus

This study by Tomoyuki Tamenori and Sakae Horisawa from Kochi University of Technology examines the cellulase-related genes in the white rot fungus Pleurotus ostreatus. The aim is to identify genes encoding endocellulases by utilizing information from cellulases of other organisms. Through bioinformatics analysis and PCR amplification with designed CODEHOP primers, we obtained amino acid sequences from biological databases. The results shed light on the genetic makeup of cellulases in this species, contributing to our understanding of lignocellulose degradation.

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Genome-Wide Survey of Cellulase-Related Genes in Pleurotus ostreatus

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  1. RG/WP Poster presenters Wood protection

  2. Poster 398 Tomoyuki TAMENORI, Sakae HORISAWA Genome-wide survey of cellulase related gene of white rot fungus, Pleurotus ostreatus Kochi University of Technology Tomoyuki Tamenori 185 Miyanokuchi, Tosayamada, City Kami-city, Kochi JAPAN

  3. Poster 398 Genome-wide survey of cellulase related gene of white rot fungus, Pleurotus ostreatus Aim of work • Genes of Pleurotus ostreatus encoding endocellulases were searched based on the information regarding the cellulases of other organisms.

  4. Poster 398 Results • Amino acid sequence data of cellulase (mainly endoglucosidase) were obtained from the biological databases. • Consensus-degenerate hybrid oligonucleotide primers (CODEHOP) were designed . • PCR amplification and sequence analysis were conducted.

  5. Poster 398 Results • PCR amplification was then performed using each pair of CODEHOPs. Sequencing

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