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Distribution of 16S rDNA Phylotypes from Lake Washington Sediments and Microcosm Experiments

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This study investigates the distribution of 16S rDNA phylotypes found in libraries derived from total RNA isolated from Lake Washington sediments and from 13C-labelled and 12C-DNA fractions obtained through microcosm experiments. Phylotypes are classified using the Ribosomal Database Project-II, with sequences defined as phylotypes exhibiting less than 97% similarity. Additionally, phylotypes lacking sufficient bootstrap confidence for classification are categorized under an "unclassified" taxon, highlighting gaps in our understanding of microbial diversity in this ecosystem.

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Distribution of 16S rDNA Phylotypes from Lake Washington Sediments and Microcosm Experiments

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  1. 16S rDNA phylotype distribution in libraries obtained from total RNA isolated from Lake Washington sediment and from 13C-labelled and 12C-DNA fractions obtained from the microcosm experiments

  2. aClassification according to the Ribosomal Database Project-II (http://rdp.cme.msu.edu/classifier.jsp); b Phylotypes were defined as sequences less than 97% similar (41). Uncl., phylotypes that could not be assigned with a bootstrap confidence estimate above the default threshold value of the RDP Classifier (80%) are displayed under an artificial ‘unclassified’ taxon

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