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Distribution of 16S rDNA Phylotypes from Lake Washington Sediments and Microcosm Experiments

This study investigates the distribution of 16S rDNA phylotypes found in libraries derived from total RNA isolated from Lake Washington sediments and from 13C-labelled and 12C-DNA fractions obtained through microcosm experiments. Phylotypes are classified using the Ribosomal Database Project-II, with sequences defined as phylotypes exhibiting less than 97% similarity. Additionally, phylotypes lacking sufficient bootstrap confidence for classification are categorized under an "unclassified" taxon, highlighting gaps in our understanding of microbial diversity in this ecosystem.

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Distribution of 16S rDNA Phylotypes from Lake Washington Sediments and Microcosm Experiments

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  1. 16S rDNA phylotype distribution in libraries obtained from total RNA isolated from Lake Washington sediment and from 13C-labelled and 12C-DNA fractions obtained from the microcosm experiments

  2. aClassification according to the Ribosomal Database Project-II (http://rdp.cme.msu.edu/classifier.jsp); b Phylotypes were defined as sequences less than 97% similar (41). Uncl., phylotypes that could not be assigned with a bootstrap confidence estimate above the default threshold value of the RDP Classifier (80%) are displayed under an artificial ‘unclassified’ taxon

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