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Biology Laboratory Course - Fall 2015

Join BIOL 171 Lab with instructor Michelle Smith focusing on measurements, biological molecules, genetics, and more. Learn through practicals, quizzes, summaries, and a research paper.

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Biology Laboratory Course - Fall 2015

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  1. BIOL 171 Lab Fall 2015

  2. Instructor: MichelleSmith Contact Information: Email: miliefsk@hawaii.edu

  3. Class Web Sites: www.wcc.hawaii.edu/facstaff/miliefsky-m/ • Syllabus • Schedule • Assignments • Handouts

  4. Lab Time & Location • Section 60086 Monday 1-3:45 • Section 60235 Monday 5:30-8:15 Imiloa 106

  5. Instructor’s Office Location & Hours Imiloa 136 xxxxx or by apt.

  6. CourseTopics Biology and diversity of: • Measurements and statistics • Biological Molecules • Microscope use and cells • Enzyme kinetics • Meiosis and mitosis • Photosynthesis • Genetics • PCR • DNA electrophoresis • Bioinformatics • Prokaryotes

  7. Cell Phones Please turn it off before arriving to class!

  8. Attendance • Mandatory • Be on Time! • Don’t expect labs to end early. • Let instructor know if you anticipate an absence.

  9. Lab Notebook • 60 pts • 4 note books lined • turned in next lab Figure 1. Frequency histogram of number of seeds per pod for koa haole, Leucaena leucocephala, seed pods. Date: 9/30/14 Lab 04 Cells Fig. 1. pseudostratified epithelia pseudostratified epithelia cillia nuclei

  10. Lab Summaries • 240 pts • 12 lab summaries (20 pts each) • Late summaries deduct 3 pts • More than 1 week late not accepted • Answer questions (typed) • Purpose, procedures, results, discussion • Be organized! Lab Summary Microscope Use

  11. Library Research Paper • 20 pts • literature searches, scientific writing, citation procedures, and bibliography formatting • 5 pages minimum • Late- 5 points deduction up to one week WCC Library citations Fritts, T.H. 1988. The brown tree snake, Boiga irregularis, a threat to Pacific islands. U.S. Fish Wildl. Serv., Biol. Rep. 88:23-25.

  12. Pre-Lab Quizzes • 13 pre-lab quizzes (15 points each) for 150 pts • Posted @ laulima • Go to BIOL 171L site • May get questions from previous lab as well as current lab • 10 best scores • No make-up quizzes • Don’t be late • Timed- 20 minutes

  13. Lab Practicals: • 2 exams • 100 points each (200 pts) • No make-up or retake Form study groups

  14. Grading • Practical Examinations (2) 200 points • Pre-Lab Quizzes (13) 150 points • Laboratory Notebook (13) 60 points • Laboratory Summaries (13) 240 points • Library Research Report 20 points TOTAL 670 points Grade cutoffs: A = above 90% B = 80%-89.9% C = 65%-79.9% D = 55%-64.9% F = below 54.9% No plus or minus final grades

  15. LabPhotoAtlas Adams, B. & Crawley, J. (2013). Van De Graff's Photographic Atlas for the Biology Laboratory (7th edition). Morton Pub. ISBN. 161731058-1 (~$40)

  16. Academic Dishonesty • Cheating • Plagiarism See student handbook on conduct code or http://www.hawaii.edu/student/conduct/imper.html

  17. Lab Safety 01

  18. Lab Policies • Sign Waiver

  19. Preparation • Read assignment before attending lab • Take pre-lab quiz up to 1 hr before lab

  20. Lab Attire You will be working with some toxic substances in lab • Lab coat • Gloves • Goggles • Proper clothing • No open-toed shoes

  21. Lab Safety Prohibited • Food • Drink • Smoking • Cell phone • Horseplay • Open-toed shoes

  22. Lab Safety • Material Safety Data Sheet (MSDS) for each chemical used is available to you upon request. • Identify the location of the fire extinguisher, first aid kit, emergency shower, and eyewash

  23. Handling Chemicals • Treat all chemicals as though they were hazardous. • Do not mouth-pipette any chemical. • Do not ingest, inhale, touch any chemical.

  24. Handling Organisms • All organisms, living or dead, should be treated with care and respect. • Some organisms may be hazardous if touched or ingested. • Avoid direct handling when possible. • Proper handling of microorganisms will be demonstrated.

  25. Lab Safety Please Report The Following to the instructor: • Injuries • Broken equipment • Broken glass or tools

  26. Lab Safety • Broken glassware, slides and coverslips must be disposed of into the “GLASS ONLY” boxes These guidelines will help to protect you from the harmful materials used in lab

  27. Lab Safety Equipment • In case of an emergency, you should know the location and proper use of all the safety equipment provided in the laboratory. • Always immediately inform the instructor of any accident.

  28. (3) Squeeze handles. (2) Pull out locking key. (1) Aim nozzle at base of fire. Fire Extinguishers

  29. Position head between spigots. Push handle to start the flow of water! Flush for at least 15 minutes.

  30. Safety Shower Any chemical spill on a person should be washed off immediately and thoroughly using the safety shower. • Pull the chain to start • the flow of water! • Rinse for 20 minutes

  31. Chemical Spills • Located on lab benches are containers of sodium bicarbonate to neutralize acid spills. These work by covering the spill of acid with a solid. • Contact your instructor immediately if there is a chemical spill. • Your instructor will handle any and all chemical spills.

  32. Protect hands. Lubricate glass surfaces. Special Caution Handling Glassware NEVER force the glassware!

  33. Waste Disposal . Discard waste as needed, during and at the end of each laboratory period

  34. Waste Disposal • Specific guidelines need to be observed for waste disposal and will be given by your teacher. • Not Sure? PLEASE ASK!!!! Waste Basket

  35. Paper Products Paper products that do not contain chemicals can be placed in the waste baskets. Waste Basket

  36. Solid and Liquid Chemicals • Solid and liquid chemicals need to be placed in appropriate containers. • The exact means will be given in the lab procedure or specified by the instructor. Never dump chemicals down the drain!Never return unused chemicals to the original stock bottle!

  37. Behavior • Have a positive attitude! • Work cooperatively with group members

  38. Clean-up • Everyone participates • Disinfect your work area • Put equipment away

  39. Most Important • Keep you eyes open to your surroundings. • If your not sure, ASK!

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