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Comparative Analysis of Phosphopeptide Quantification in Cell Lines HEL and MV4

This supplementary figure presents the mean coefficients of variation (CV) for phosphopeptide quantification across HEL and MV4 cell lines. The analysis reveals a consistent log2 fold change with values of 18.8% for P31/Fuj versus MV4, and 13.5% for HEL versus MV4. The overall mean CV across all measured phosphopeptides is noted as 28.1%. This data highlights the variation in phosphopeptide abundance as determined by mass spectrometry, indicating the reliability and reproducibility of the quantification across different charges.

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Comparative Analysis of Phosphopeptide Quantification in Cell Lines HEL and MV4

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  1. Supplementary Figure 7 P31/Fuj Vs MV4 Mean CV = 18.8 % Log2 Fold HEL Vs AML-193 HEL Vs MV4 Mean CV = 13.5 % Mean CV = 28.1 % Log2 Fold Quantification of phosphopeptides identified with more than one charge. Mean CV refers to the mean coefficient of variation of the fold difference for phosphopeptides quantified with more than one charge across the cell lines shown.

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