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Tips to Perform Pipette Calibration

Pipettes are necessary equipment often used in chemical laboratories to measure and transfer specific amount of liquid.

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Tips to Perform Pipette Calibration

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  1. Tips to Perform Pipette Calibration • Pipettes are necessary equipment often used in chemical laboratories to measure and transfer specific amount of liquid. • Pipettes are essentially narrow tube like equipment with a rubber bulb at the top. • The tube is numbered from the top to the bottom mostly in increments of ten millimeters. • Accuracy in pipette measurement is necessary as any discrepancy in reading can affect the result of chemical reaction. • To ensure accuracy it is necessary to perform pipette calibration at regular intervals. • Calibration of equipment is desirable even in the most advanced equipment as overtime several factors can affect the readings of measuring equipment. • Calibration process helps to check whether or not the equipment is offering correct reading so that it can be rectified in time and efficiency of processes or laboratory test is maintained.

  2. Given below are instructions to help you perform pipette calibration on your own. • Things needed: • Beaker • Erlenmeyer flask • Pen and paper • Pipette • Pipette filler • Thermometer • Weight balance

  3. 1.Clean the Pipette : 2. Add Distilled Water: Find out the mass of the beaker to the nearest tenth of a milligram, using a balance. • Put some distilled water in an Erlenmeyer flask. Let it stand out on a bench for about 15 minutes. Now, measure the temperature of the water.

  4. 3. Taking Help of Pipette Filler: Calculate and determine: Calculate and determine the mean of the four pipette measurements. • Now, using a pipette filler fill the pipette with the water from the flask. Move this water into the beaker. Weigh the beaker again. Note down the difference in weight of the beaker and calculate the mass of water discharged. Carry out this process three more times.

  5. 5. Adjusting the Buoyancy of Air: 6. Calculating Density of Water: Calculate the density of water at the temperature you measured earlier in the process. Determine the mean volume of water discharged by the pipette using the formula: Volume = mass / density. • Make an addition of 1.06 mg per gram to the mean mass to adjust for the buoyancy of air during the weighing. Do not follow this step in case you are using a digital scale.

  6. It is important to ensure your laboratory is clean and organized when carrying out calibration.

  7. You are not required to clean and dry the beaker following each of the four pipette measurements as doing this will only waste your time.

  8. Take help of pipette calibration service provider • Even after taking precautions and doing all the steps correctly, there are chances that your calibration process may not offer you precision in results. Besides, you may not like to use your valuable resources for this purpose. A great way to help you carry out pipette calibration work with accuracy is to take help of professional pipette calibration service providers. Certified agencies keep an array of sophisticated equipment and have a team of experience technicians to conduct calibration of equipment with precision and care, thereby helping you carry out laboratory tests with efficiency.

  9. For Further Info Visit www.RScalibration.com

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