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Transmission Electron Micrographs and Lipid Analysis of Lacinutrix Species

This document presents supplementary figures featuring transmission electron micrographs of Lacinutrix jangbogonensis PAMC 27137T (a), Lacinutrix mariniflava JCM 13824T (b), and Lacinutrix algibacter JCM 13825T (c), all cultivated on marine agar at 10°C. Additionally, it includes two-dimensional thin layer chromatograms of total polar lipids from these species, analyzed after spraying with molybdato-phosphoric acid. Key lipids identified include phosphatidylethanolamine (PE) and several unidentified aminolipids and lipids, using various staining techniques.

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Transmission Electron Micrographs and Lipid Analysis of Lacinutrix Species

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  1. Supplementary Fig. S1. Transmission electron micrographs of Lacinutrixjangbogonensis PAMC 27137T (a), LacinutrixmariniflavaJCM 13824T (b), andLacinutrixalgibacterJCM 13825T (c). Cells were grown on marine agar at 10C. (a) (b) (c)

  2. Supplementary Fig. S2. Two-dimensional thin layer chromatograms of the total polar lipids of Lacinutrixjangbogonensis PAMC 27137T (a), LacinutrixmariniflavaJCM 13824T (b), andLacinutrixalgibacterJCM 13825T (c), after spraying the plate with molybdatophosphoric acid. PE, phosphatidylethanolamine; AL1-AL2, unidentified aminolipids; APL, unidentified aminophospholipid; L1–L6, unidentified lipids. Lipids were identified as phospho- or amino- by spraying with molybdenum blue and ninhydrin, respectively.  L1 L2 PE APL (a) (b) (c) AL1 L3 L4 L5 L1 L6 L2 PE AL2 AL1 L3 L4 L5 L1 L2 PE AL1 L3 L4

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