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Eukaryotic Translation

Eukaryotic Translation. mRNA tRNA rRNA. mRNA. -monocistronic -1,000-2,000 bases long -methylated at 5’cap -100-200 bases of poly A at 3’end -nontranslated 5’ reader < 100 bases -nontranslated 3’ tailer ~ 1,000 bases - AUG as the INITIATOR -UAA,UAG,&UGA as TERMINATOR.

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Eukaryotic Translation

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  1. Eukaryotic Translation mRNA tRNA rRNA

  2. mRNA -monocistronic -1,000-2,000 bases long -methylated at 5’cap -100-200 bases of poly A at 3’end -nontranslated 5’ reader < 100 bases -nontranslated 3’ tailer ~ 1,000 bases - AUG as the INITIATOR -UAA,UAG,&UGA as TERMINATOR

  3. Fig5.16 Expression of mRNA

  4. Life Cycle of Eukaryotic mRNA -removal of Poly(A) need for decapping enzyme -Poly(A)-binding protein, PABP on Poly(A) prevents the decapping enzyme from binding to the 5’end

  5. AU-rich sequence, called ARE ~50 bases in 3’ tailer -AUUUA, more repeated -trigger destabilization

  6. TRE sequence - contain strm-loop structures for protein binding -Protein inhibit destabilizing

  7. tRNA Met-tRNAifor initiation Met-tRNAmforelongation - No formylated Methionine -has an unusual tertiary structure, modofied by phosphorylation of the 2’ribose position

  8. Eukaryotic Ribosome

  9. The right context for Recognization -AUG -the most important seq. GCCAGCCAUGG, purine three bases before AUG and G immediately following it. CAP; for a stable complex to be formed at initiation codon

  10. Eukaryotic Initiation *form ternary complex *bind free 40S *attach the 5’ end Ternary complex; -Met-tRNAi -eIF2 -GTP

  11. Initiation Factors *bind 5’ cap *unwind mRNA *bind subunit initiation complex *support 60S joining

  12. Elongation Factors: EF-Tu, load aminoacyl tRNA EF-Ts, GDP to GTP

  13. Translocation: Peptidyl transferase, synthesis peptide

  14. EF-Tu EF-G

  15. Fig.6.27

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