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Microscope Slide Preparation Guide: Wet Mount Technique

Learn the step-by-step procedure for preparing a wet mount slide using a glass slide, specimen, coverslip, and water. Ensure proper handling to view specimens clearly under the microscope.

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Microscope Slide Preparation Guide: Wet Mount Technique

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  1. The Wet Slide

  2. The Players • Slide • Specimen • Coverslip • Water

  3. The Slide • Made of Glass • Sharp • Fragile

  4. The Specimen • Small • Fragile • Potentially harmful

  5. The Coverslip • Plastic • Small • Fragile

  6. Water • Wet • One drop only

  7. Procedure • Obtain the slide

  8. Procedure • Place specimen in the center of the slide

  9. Procedure • Place one drop of water on the specimen

  10. Procedure Place Slide cover perpendicular to the slide Slowly allow slide cover to drop over the specimen

  11. Procedure Place Slide cover perpendicular to the slide Slowly allow slide cover to drop over the specimen

  12. Procedure Place Slide cover perpendicular to the slide Slowly allow slide cover to drop over the specimen

  13. Procedure Place Slide cover perpendicular to the slide Slowly allow slide cover to drop over the specimen

  14. Viewing Start on Low power Use Coarse Adjustment knob to find the specimen Use fine Adjustment knob to focus

  15. Viewing Change to Medium Power Use FINE Adjustment knob to focus

  16. Viewing Start on high power Use FINE Adjustment knob to focus

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