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ProStar 410 Autosampler

ProStar 410 Autosampler. P. van Poppel – Field Support Specialist. October 2009. Overview. General Description Specifications Flow path Injection principles Subassemblies Service. 13. 1. 12. 11. 2. 10. 9. 3. 4. 5. 8. 6. 7. General Description. Needle arm Injection valve

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ProStar 410 Autosampler

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  1. ProStar 410 Autosampler P. van Poppel – Field Support Specialist October 2009

  2. Overview • General Description • Specifications • Flow path • Injection principles • Subassemblies • Service

  3. 13 1 12 11 2 10 9 3 4 5 8 6 7 General Description • Needle arm • Injection valve • Oven compartment • Tubing guide • Wash position • Transport vials or reagent A-B • Wash & Waste • Condensed & leakage • Tray fixation • Vial positions 12 mm • Syringe dispenser • Keyboard • Buffer tubing

  4. In- and Outputs • 1. TTL Input Connector • Next injection • Freeze • Stop • 2. Contact closure output • 2x Inject Marker • 2x Aux • Alarm • 3. Serial Interface connector • RS 232 MultiLink

  5. Specifications • Std. 84 x 1.5 mL vials + 3 x 10 mL vials • Loopvolume 1-5000 µL • Syringe 250 µL or 1000 µL • Vial detection • Headspace pressure 7PSI (0.5Bar) • Inj. Valve switching time <100 msec • Column heater (Tambient + 5°C) to 60 ºC

  6. Buffer Tubing Syringe Valve Syringe Injection Valve Needle arm Air Needle Injection Needle Flow Path From Pump To Column

  7. PASA™: Pressure Assisted Sample Aspiration

  8. Injection Principles • Full loop injections • Partial loopfill injections (up to 50% of loop volume) • µL pick-up injections

  9. Pre-flush Fill the sample supply line quantitatively inject

  10. Pre-flush Switch valve to Loadand cut off the diluted front load

  11. Full loop injection Unrivalled precision !Fill the sample loop load

  12. Full loop injection Switch valve to Inject inject

  13. Partial loopfilling Programmable injection volumes down to micro-volumes load

  14. Partial loopfilling Switch valve to Inject inject Max. injection Volume: 50% of sample loop

  15. Sample consumption • Full loopfill modeLoop = 20 µLStd sample needleTotal sample consumption:…………...? • Partial loopfill modeInj. Volume = 10 µLStd sample needleTotal sample consumption: …………..?

  16. Sample consumption • Full loopfill modeLoop = 20 µL 3 x 20 = 60 µLStd sample needle 2 x 15 = 30 µLTotal sample consumption: 90 µL • Partial loopfill modeInj. Volume = 10 µL 1x10 = 10 µLStd sample needle 2 x 15 = 30 µLTotal sample consumption: 40 µL

  17. Flow Profile needle tubing loop buffer tubing

  18. µL Pick-up Zero sample lossFlush with transport solvent inject

  19. µL Pick-up Pick-up sample load

  20. µL Pick-up Transport sample to the center of the loop load Max. Injection volume: (Loopvolume - 3 x needle volume) / 2

  21. µL Pick-up Switch valve to Inject inject

  22. System Settings General • Loop volume 1-5000 µL • Needle volume 15 or 45 µL • Syringe volume 250 or 1000 µL • Syringe speed • Skip missing vials • Air segment • Headspace pressure

  23. System Settings Method / Tray • Use Timed events • Use Mix • Use calibration vials • Tray type: • 96 • 84 + 3 • 24

  24. System Settings • I/O • Inject marker pulse length • Input edge next injection • Freeze input active • Reset outputs after last series • Serial • Device Identifier 60 - 69

  25. Injection Methods • Injection type: • Full Loop • Partial loop • µL Pick-up • Wash • Time events • Mix

  26. Injection Method Programming

  27. Mix method • Add 100 µL from Reagent A to Destination • Add 200 µL from Reagent B to Destination • Add 500 µL from Sample to Destination • Number of Mix cycles: 3volume 600 µL • wait: 0:02:30 • End of Mix method

  28. Time based Method • 1 Aux 1 ON at time: 0:00:10 • 1 Aux 1 OFF at time: 0:02:15 • 1 Aux 2 ON at time: 0:04:00 • 1 Aux 2 OFF at time: 0:05:59 • Initial oven setpoint at 60 ºC • 1 oven setpoint 50ºC at time 0:10:00 • End of timed events at: 0:30:00

  29. Series • Series number 1-9 • Method number 1-9 • First Sample vial • Last Sample vial • Calibration vials* • First Destination vial* * Depends on the System settings

  30. Options • Peltier tray cooling option • Bio Injection valve • Large capacity option • Large Sample Volume option

  31. Prep-Mode • Large bore VALCO Injection Valve • Syringe of 2.5 mL • Buffertubing 2000 µL • Default Sample loop of 10 mL (max. 20 mL) • Inj. vol. max:19999 µL • Available as: factory installable and Field Prep-kit!!

  32. Needle Construction • Ferrule • Nut • Tefzel tubing • Nut • Teflon ferrule • Conical ring • SS Sample Needle • Part of Silicon tubing • Needle body • Air Needle • Vial feeler assy • Hex screw (Needle height) • Tygon tubing

  33. Needle body Needle height adjustment screw Needle Height Adjustment • Set the needle body as high as possible. • Perform an injection in a vial with the highest bottom. • Adjust the needle height.

  34. Main-Board EPROM • 68000 CPU Processor • Integrated Power Supply • Easy access to Firmware

  35. A A Injection Valve Assembly • Customer removable Valve. • Easy Stator and Rotorseal replacing. • Stepper motor driven • Stainless Steel and Peek Valves • Rotorseals made of: • Tefzel • Peek • Vespel • Valcon H • Valcon E

  36. Valve body Driver shaft Rotor seal Stator Stator screws Valco Injection Valve

  37. Valve Drive Assembly

  38. Removing the Dispenser

  39. Removing Dispenser Valve

  40. 5 4 6 3 7 1 8 2 Syringe Unit • Fluid displacement stepper motor • Syringe • Syringe valve stepper motor • Optical control of valve • Syringe valve sensor disk • Rotation sensor • Home sensor • Home vane

  41. Syringe Home Adjustment

  42. Penetration Point Adjustment Wrong penetration points

  43. Needle Penetration Adjustment • Left-RightRotate the Needle-Unit

  44. Needle Penetration Adjustment • Tray stop position

  45. Setscrew Vial Stripper • Adjust the stripper with the Setscrew

  46. Vial Sensor • Distance between sensor and tray: 1.5 mm 1.5 mm

  47. Vial Sensor Replacement

  48. 0 1 0 4 6 6 E Service Mode Autosampler [MENU] READY (2.07) OVEN:--°C TRAY:--°C <WASH> <MAINTENANCE> <SERIAL> <TEMP> Autosampler [MENU] READY (2.07) OVEN:--°C TRAY:--°C <Utils> <SERVICE> ENTER SERVICE-CODE, THEN PRESS ENTER

  49. Tray Diagnostic SERVICE MODE TRAY SEARCH VIAL:XX SENSOR HOME:X POS:X TYPE:84+3 <START>

  50. Needle Diagnostics SERVICE MODE [MENU] NEEDLE VERTICAL SENSORS UP:X DOWN:X VIAL:X <HOME> <DOWN> SERVICE MODE [MENU] NEEDLE HORIZONTAL SENSORS UP:X LEFT/RIGHT:X WASH:X <HOME> <NEXT> <PREVIOUS> SERVICE MODE [MENU] NEEDLE COMPRESSOR <ON> <OFF>

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