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Blood and Hemopoiesis Prof. Ji-Cheng Li (Zhejiang University School of Medicine)

Blood and Hemopoiesis Prof. Ji-Cheng Li (Zhejiang University School of Medicine). 1. Components: ---formed elements: 45% red blood cell- erythrocyte white blood cell- leukocyte platelets. ---plasma: 55%, PH 7.3-7.4, 90% water contain:

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Blood and Hemopoiesis Prof. Ji-Cheng Li (Zhejiang University School of Medicine)

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  1. Blood and HemopoiesisProf.Ji-Cheng Li(Zhejiang University School of Medicine)

  2. 1.Components: ---formed elements: 45% • red blood cell-erythrocyte • white blood cell-leukocyte • platelets

  3. ---plasma: 55%, PH 7.3-7.4, 90% water contain: plasma protein (albumin, globulin, fibrinogen), lipidprotein enzymes hormone vitamin inorganic salt products of metabolism serum: plasma without fibrinogen

  4. Blood smear: Wright or Giemsa stain methods

  5. 1.   Cells

  6. 1) erythrocyte, red blood cell ---normal number: (4.2-5.5)X1012/L in male; (3.5-5.0)X1012/L in female ---structure: • biconcave discs in shape, • 7.5-8.5 um in diameter, 1-2um thick • no nucleus and organella • filled with hemoglobin (Hb)

  7. -normal Hb: 120-150g/L in male 105-135g/L in female -Function: combine and transfer Oxygen (O2) and Carbon dioxide(CO2)

  8. reticulocyte: • immature cell • remained ribosome: delicate network structure when stained with brilliant cresyl blue • 0.5-1% of total erythrocyte population in adult • 3-6% in neonate ---life span: 120 days

  9. 2) leukocyte ---a group of large cells with nucleus ---involve in defense and immune reaction ---normal number: (4.0-10) X109/L ---classification: • granulocytes: /neutrophil /eosinophil /basophil • agranulocytes: /lymphocyte /monocyte

  10. ①neutrophil: 50-70% of total leukocyte population ---structure: LM: • round, 10-12um in diameter • rob-liked or polymorphous nucleus, 2-5 lobes • fine neutrophilic granules • pink cytoplasm

  11. EM: specific granule: • 80% • small, 0.3-0.4 um ovoid or irregular in shape • contain: lysozyme, phagocytin (defensin) azurophilic granule: • 20% • large, round or ovoid , 0.6-0.7 um • electron dense • contain: acid phosphatase, peroxidase, ect.

  12. ---function: phagocytose bacterium: • specific g.-kill B • azurophilic g.- digest B ---life span: in blood 6-8 hours, in CT 2-3 days

  13. Neutrophilic granulocyte Neutrophilic granulocyte

  14. ②eosinophil: 0.5-3% of total leukocyte population LM: • round,10-15 um in diameter • 2 lobes nucleus • rough bright red granules: acidophilic granules

  15. EM: granules: • round or ovoid • with cube-liked electron dense crystal • contain: -ACPase -histaminase -peroxidase

  16. ---function: counteract the infection of parasite reduce allergic reaction ---life span: in blood 6-8 hour, in CT 8-12 days

  17. Eosinophilic granulocyte 致密结晶体

  18. ③basophil: 0-1% of leukocyte population LM: • less, round 10-12 um • lobe, “S” shaped or irregular nucleus • basophilic g.: contains heparin, histamine and acidophil chemotactic factor • cytoplasm: contain leukotriene ---function: involve in allergic reaction ---life span: 10-15 days

  19. Basophilic granulocyte

  20. ④     monocyte: 3-8% of total leukocyte population LM: • large round, 14-20um • nucleus appear as kidney, horse-shoe or ovoid in shape • cytoplasm: gray-blue in color, contain azurophilic g.

  21. EM: • azurophilic granule: peroxidase, ACPase, lysozyme ---function: actively mobile and chemotaxis ---life span: in blood 1-5 days, in CT which become into macrophage- mononuclear phagocytic system

  22. ⑤ lymphocyte: 20-30% of total leukocyte population LM: • round, small LC 6-8 um, medium-sized LC 9-12 um, large LC 13-20 um in diameter • round nucleus with indentation, chromatin appears as spot-liked and electron-dense • cytoplasm: /basophilic:bright blue in color /less /azurophilic granule

  23. EM: /free ribosome /mito. /RER ---classification: • TC: thymus dependent lymphocyte, 75%, involve in cellular immune reaction and regulate immune response • BC: bone marrow dependent lymphocyte, 10-15%, become into plasma cell, involve in humoral immune response • large granular cell: KC (killer cell) and NKC( nature killer cell), 10% ---function: involve in immune response

  24. Lymphocyte

  25. division

  26. 3) blood platelet: cytoplasmic fragment of megakaryocyte in bone marrow ---normal number: 100-300X109/L ---structure: LM: • disc-liked, 2-4um → irregular in shape Stimulation • in groups • Granulomere • hyalomere

  27. EM: /tubular systems: • opening tubule system- increasing the exchange area, facilitate the intaking and releasing • dense tubule system- SER, collecting calcium (Ca2+ ) and synthesizingprostaglandin /granules: • specific granule: mediate electron density, with dense core, contains clotting factor,fibrinogen, acidic hydrolase • dense granule: electron dense, contains serotonin(5-hydroxytryptamine), ADP,ATP, Ca2+ and adrenalin

  28. ---function: • involve in clotting and stopping the bleed: -aggregation -release components of granule, make thrombinogen become into thrombin, then later make fibrinogen become into fibrin • protecting endothelium and take part in repairing of endothelium

  29. 血小板（EM） Blood platelet 特殊颗粒

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