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PAM Fluorometry: Measuring Photosynthesis

PAM Fluorometry: Measuring Photosynthesis. Brian Maniaci. Photosynthesis. 6 CO 2 + 12 H 2 0 + light Energy  C 6 H 12 O 2 + 6 O 2 + 6 H 2 0. Phytoplankton. Photosynthetic organism Chlorophyll A Euphotic Layer Less than 5 mm long. Marine Food Chain. Energy . Use of light energy

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PAM Fluorometry: Measuring Photosynthesis

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  1. PAM Fluorometry: Measuring Photosynthesis Brian Maniaci

  2. Photosynthesis • 6 CO2 + 12 H20 + light Energy  C6H12O2 + 6 O2 + 6 H20

  3. Phytoplankton • Photosynthetic organism • Chlorophyll A • Euphotic Layer • Less than 5 mm long

  4. Marine Food Chain

  5. Energy • Use of light energy • Photosynthesis • Heat • Fluorescence • Fluorescence –emission of light at a lower energy/higher wavelength

  6. Measuring Photosynthesis • Reactants • Radioactive Carbon-14 • Products • Winkler’s Method-O2 Concentration • PAM Fluorometer • Fluorescence of emitted Light

  7. PAM Fluorometer

  8. PAM Fluorometer • Pulse amplitude modulated fluorometer • Chlorophyll fluorescence • Ideal environment • Sensitive and noninvasive

  9. PAM 101 Unit • 3 mW laser diode • Photodiode detector • Fresnel lens • Distance 0.5- 1 meter

  10. Laser Diode • Promotes fluorescence • Dual lasers to generate homogenous spot • Emits light at 638 nm. • Return signal magnified through Fresnel Lens

  11. Quantum Yield • Definition: • ∆F = Fmax –Fmin of fluorescence • Fm = Fmax of fluorescence • ∆F/Fm = Quantum Yield • Quantum yield of effective photosynthetic electron transport (PS 1)

  12. Characteristics • Extremely sensitive • Measuring system • Can measure a single cell, chloroplast or macroalgae • Doesn’t change physiology of the organism

  13. Chlorophyll Fluorescence Mapping • Blue LED (470 nm) used for excitation • Measured at 665 nm

  14. Conclusion • Photosynthesis • Organism • Ideal Environment • Comparison of scientific data

  15. Acknowledgements • Dr. Moline • Esteemed Colleagues

  16. References: • "Algal Growth." A. Encyclopedia of Life Sciences. 1 vols. New York: Nature Group, 2002. • Grunwald, B, and M Kuhl. "A System for Imaging Variable Chlorophyll Fluorescence of Aquatic Phototrophs." Ophelia 58 (2004): 79-89. 28 Feb. 2008. • Ounis, A, S Evian, S Tosti, and I Moya. "Adaption of PAM-Fluorometer for Remote Sensing of Chlorophyll Fluorescence." Photosynthesis Research 68 (2001): 113-120. 28 Feb. 2008.

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