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Animal Science 434

Animal Science 434. Lecture 15: Gamete Transport. Muscle Contractions. Cilia Fluid Flow. Fluid Flow. Sperm Transport in the Male Tract. Sperm Transport in the Male Tract. Acquisition of Fertilizing Ability and Motility. No protein synthetic ability

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Animal Science 434

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  1. Animal Science 434 Lecture 15: Gamete Transport

  2. Muscle Contractions Cilia Fluid Flow Fluid Flow Sperm Transport in the Male Tract

  3. Sperm Transport in the Male Tract Acquisition of Fertilizing Ability and Motility • No protein synthetic ability • addition or loss of plasma membrane proteins and lipids • redistribution of proteins and lipids within sperm • changes in lipid diffusion coefficients

  4. Addition of DF From Accessory Gland Secretions Sperm Transport in the Male Tract Addition of Decapacitation Factor • Decapacitation Factor • protein, glycolipid and/or lipid • stabilizes the plasma membrane

  5. Site of Ejaculation Site of ejaculation Semen Characteristics Species Vagina slight coagulation of ejaculate human, rabbit vagina semen with high sperm cattle, sheep concentration uterus voluminous, distention of cervix horse cervix, uterus voluminous, retention of penis dog, pig during copulation uterus vagina plug rodents

  6. Sperm Loss From Female Tract • retrograde flow • phagocytosis by neutrophils

  7. Experiment on Retrograde Flow in the Cow Uterine Horn Insemination at 3 different spots Uterine Body Cervix

  8. Uterine Body Uterine Horn 20 15 Cumulative % Sperm Recovered From the Vagina 10 Cumulative % Sperm Recovered From Vagina 5 0 0 1 2 3 4 5 6 7 8 Hour After Insemination

  9. Cervix Uterine Horn 60 50 40 Cumulative % Sperm Recovered From the Vagina 30 cervix 20 10 0 0 1 2 3 4 5 6 7 8 Hours After Insemination Hour After Insemination

  10. Evolutionary Strategies for Dealing With Retrograde Flow of Semen • coagulation of semen or a plug • concentrated semen • semen deposited in uterus

  11. Sperm Loss From Female Tract • retrograde flow • phagocytosis by neutrophils

  12. Neutrophile Infiltration of Uterus

  13. Phases of Sperm Transport • rapid • 15-30 min • sustained • 6 - 12 hr • oviductal • at ovulation

  14. Evidence for Sustained Transport Ligatures at Utero-tubal Junction

  15. Effect of Oviduct Transection on Fertilization in Heifers Interval from mating* to Oocytes Fertilized transection of UTJ 6 0% 8 50% 12 83% *Mating was done at the start of estrus.

  16. Sperm Transport in the Pig Percentage of Oocytes Fertilized }

  17. Ovulation Oocyte Cumulus Cells Follicular Fluid

  18. Cue for ovulation may effect isthmus to trigger oviductal transport

  19. 18-24 hr 1-2 hr 6-12 hr Location of Fertilizing Sperm

  20. Barriers To Sperm Transport

  21. Cervix

  22. Barriers To Sperm Transport Uterotubal Junction

  23. Ovulation Oocyte Cumulus Cells Follicular Fluid

  24. Remains closed until day 3 - 5 post ovulation Factors Regulating Oocyte Transport in the Oviduct Fluid Flow

  25. Total Fluid Production Toward Ampulla Toward Uterus

  26. Factors Regulating Oocyte Transport in the Oviduct Site of Fertilization No Contractions Cilia Muscle Contractions Fluid Flow Remains closed until day 3 - 5 post ovulation

  27. Now view the videopodcast entitled Ovulation and Oocyte Transport Obtain from Lean@UW podcast links for this course in the Lecture area.

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