1 / 1

Analysis of PEXEL-Tagged Plasmodium berghei Schizonts Using mCherry and GFP Markers

This study examines the expression and localization of PEXEL-tagged Plasmodium berghei schizonts, utilizing mCherry and GFP fluorescence markers. We analyzed the genetic expression of pbanka_136550 and its impact on parasite development at several time points post-infection. Using Hoechst staining to visualize nuclei, we distinguished between uninfected and infected red blood cells, and quantified the fluorescence levels of schizonts at varying stages—ring, trophozoite, and mature forms. Statistical significance was determined with p-values less than 0.0001.

evangeline-byrd
Télécharger la présentation

Analysis of PEXEL-Tagged Plasmodium berghei Schizonts Using mCherry and GFP Markers

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. G1 G2 Hoechst GFP Supp Figure 5 A PEXEL PBANKA_136550 327aa mCherry TM B pbanka_136550::mCherry pbanka_136550 ::mCherry ANKAwt Chr 13- G1 G2 95%(1%) anti- mCherry ~100kDa Hoechst Chr 7- Uninfected rbc ~70kDa ctrl 3’utr dhfr mCherry C pbanka_136550 ::mCherry schizont 20h schizont 24h ring 4h troph. 16h troph. 12h D Chr 13- Δ pbanka_ 136550 ANKAwt 1x107 5x106 ~1,5kb IBIS1 Chr 7- ctrl Chr 3- Δpbanka_136550 3’utr dhfr Δ pbanka_136550 K173cl1-GFP-Lucschiz -a Δ pbanka_136550 P<0.0001 P<0.0001 GFP+-schizonts (%) – G2 all schizonts (%) – G1 Hours post infection

More Related