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Concentration-Dependent NF-κB Analysis Using Real-Time PCR Amplification Data

This supplemental data figure presents the concentration-dependent relative Ct values (∆Ct) of NF-κB. A linear regression fit (R² = 0.999) illustrates the relationship between NF-κB concentration (ranging from 10 fM to 2 nM) and ∆Ct values. Included are amplification plots from real-time PCR for the 2 nM NF-κB probe, demonstrating the effect of varying NF-κB concentrations on detection sensitivity. This data aids in understanding NF-κB dynamics and its role in cellular signaling pathways.

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Concentration-Dependent NF-κB Analysis Using Real-Time PCR Amplification Data

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  1. ∆Ct + 7 1 - NF-κB concentration (fM) Supplemental Data Fig. 2. NF-κB concentration-dependent relative Ct values (∆Ct). The solid red line is a linear regression fit (R2 =0.999) to the ∆Ct values. Insert is the amplification plots of real-time PCR for the 2 nM NF-κB probe in the presence of increasing amounts of NF-κB. The final concentrations of NF-κB were 10 fM (curve 1), 100 fM (curve 2), 1 pM (curve 3), 10 pM (curve 4), 100 pM (curve 5), 1 nM (curve 6), and 2 nM (curve 7), respectively. ‘+’ and ‘-’ indicate positive and negative controls, respectively.

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