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Bio 21 Fall 2005 Lab 1 9

Please read Standard Curve Lab instructions before coming to class Thursday.Read, sign, and turn in the two pages of Academic Honesty Agreement and Safety Rules Acknowledgement by Thursday, 9/1Purchase five Scantron form No. 882-E and turn them in by next Tuesday, 9/6 (do not write your name). GO

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Bio 21 Fall 2005 Lab 1 9

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    1. Bio 21 Fall 2005 Lab #1 9/1/05 Standard Curve Preparation

    2. Please read Standard Curve Lab instructions before coming to class Thursday. Read, sign, and turn in the two pages of Academic Honesty Agreement and Safety Rules Acknowledgement by Thursday, 9/1 Purchase five Scantron form No. 882-E and turn them in by next Tuesday, 9/6 (do not write your name)

    3. GOOD LABORATORY PRACTICE Please read Lab Safety Rules. Please handle the cuvettes and other lab instruments with care, e.g., do not slam close the sample cover. Clean up everything that you used on your bench. Do not forget to take the cuvette out of the spec when done. Dispose of wastes in proper containers

    4. LAB #1 INTRODUCTION pp. 1-2 SPECTROPHOTOMETER pp. 2-6 ERROR ANALYSIS pp. 6-9 STANDARD CURVE PREP pp. 10-12 CONSTRUCT FIGURES AND WRITING LAB REPORT pp. 12-15 TIPS ON WRITING LAB REPORTS pp. 16-19 Two Figures due 9/6/05 Lab Report #1 due 9/13/05

    5. Applications Protein/DNA determination Enzyme assay Immunoassay/ELISA Radioimmunoassay Virtually all clinical assays

    6. A Standard Curve

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