1. Yoon et al. Supplemental Figure S1 A B AGS MKN-45 N87 CFPAC-1 PANC-1 AGS MKN-45 N87 CFPAC-1 PANC-1 Monolayer Monolayer Monolayer Monolayer Monolayer Monolayer Monolayer Monolayer Monolayer Monolayer Spheroid Spheroid Spheroid Spheroid Spheroid Spheroid Spheroid Spheroid Spheroid Spheroid AGS MKN-45 N87 CD24 Spheroid media Spheroid media Spheroid media CD44 Monolayer Monolayer Monolayer CD133 β-actin 4 hr 4 hr 24 hr 24 hr 72 hr 72 hr 1 hr 4 hr 24 hr 72 hr 1 hr 1 hr β-actin C D Shh Ptch1 Smo Gli1 β-actin N87 AGS MKN-45 E Monolayer media Monolayer media Monolayer media 1 hr 4 hr 24 hr 72 hr 1 hr 4 hr 24 hr 72 hr 1 hr 4 hr 24 hr 72 hr Gli1 CD44 β-actin