1 / 12

Polymerase chain reaction AMPLIFYING DNA

Polymerase chain reaction AMPLIFYING DNA. What do you need to replicate DNA?. http://www.youtube.com/watch?v=uDumZT5z5R8. http://www.dnalc.org/view/15475-The-cycles-of-the-polymerase-chain-reaction-PCR-3D-animation-with-no-audio.html. Which DNA SEQUENCE IS AMPLIFIED?. Tandem repeats (VNTR).

fay
Télécharger la présentation

Polymerase chain reaction AMPLIFYING DNA

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Polymerase chain reactionAMPLIFYING DNA • What do you need to replicate DNA? http://www.youtube.com/watch?v=uDumZT5z5R8

  2. http://www.dnalc.org/view/15475-The-cycles-of-the-polymerase-chain-reaction-PCR-3D-animation-with-no-audio.htmlhttp://www.dnalc.org/view/15475-The-cycles-of-the-polymerase-chain-reaction-PCR-3D-animation-with-no-audio.html

  3. Which DNA SEQUENCE IS AMPLIFIED?

  4. Tandem repeats (VNTR)

  5. Tandem repeat genes 5’TTAACCTTAACCTTAACCTTAACCTTAACCTTAACC3’ 5’TTAACCTTAACCTTAACC 3’

  6. Determining your allelescreating dna fingerprint using gel electrophoresis

  7. Sample gel 5’TTAACCTTAACCTTAACCTTAACCTTAACCTTAACC3’ 5’TTAACCTTAACCTTAACC 3’ Ladder Suspect 1 Suspect 2 Crime Scene

  8. How unique are you?

  9. RflpRestriction fragment length polymorphism

  10. EXAMPLE USE TTCC RESTRICTION ENZYME DNA SAMPLE ONE: ACTGTTCCACACTTCCGCACATCTTAATTCC DNA SAMLE TWO: GTTCCATGCCGAGTACGTTCCATGCTA

  11. I am your father….well maybe

More Related