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VCAM-binding Peptide as a Contrast Agent for High-Resolution MRI Imaging

This study explores the application of a VCAM-binding peptide with USPIO contrast agents for enhanced magnetic resonance imaging (MRI) targeting of leukocyte infiltration in vascular diseases. Utilizing photon emission and molecular energy shifts, the technology allows for high-resolution imaging of various tissues, revealing intricate details about endothelial cells, smooth muscle cells, and immune response dynamics. Various tissue samples, including liver, spleen, and lung, were analyzed before and after injection to evaluate the effectiveness of the imaging technique in tracking disease progression.

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VCAM-binding Peptide as a Contrast Agent for High-Resolution MRI Imaging

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  1. Michalska, ATVB, 2012, online

  2. Leukocyte VCAM (on EC) Endothelial Cells Intima Smooth Muscle Cells http://www.clinchem.org/content/54/1/24/F1.expansion.html

  3. P03011 VCAM-binding peptide USPIO (Contrast Agent for MRI) (targeting motif) http://nihlibrary.ors.nih.gov/ipdcdb/ipdcdb_main.asp?Page=4

  4. SECARS? • Light hits molecule • Molecule energy is shifted up to virtual energy state • Photon emitted when molecule drops down in energy • Anti-Stokes is when energy is less than original • Allows for high resolution imaging http://en.wikipedia.org/wiki/Raman_spectroscopy

  5. t1/2 = 4.0±2.4h t1/2 = 7.2±2.5h t1/2 = 5.0±1.7h t1/2 = 6.0±1.3h

  6. Spleen pre-injection Spleen post-injection DAPI CD68 Liver pre-injection Liver post-injection Prussian blue stains iron Lymph post-injection Lung post-injection kidney post-injection muscle post-injection

  7. Michalska, ATVB, 2012, online

  8. 12 wk 30 wk Michalska, ATVB, 2012, online

  9. Michalska, ATVB, 2012, online

  10. early plaques late plaques Control injection Michalska, ATVB, 2012, online

  11. Michalska, ATVB, 2012, online

  12. Chen, Biomaterials, 2009, 30, 79-88

  13. Chen, Biomaterials, 2009, 30, 79-88

  14. Chen, Biomaterials, 2009, 30, 79-88

  15. Chen, Biomaterials, 2009, 30, 79-88

  16. Chen, Biomaterials, 2009, 30, 79-88

  17. Chen, Biomaterials, 2009, 30, 79-88

  18. Chen, Biomaterials, 2009, 30, 79-88

  19. Chen, Biomaterials, 2009, 30, 79-88

  20. Chen, Biomaterials, 2009, 30, 79-88

  21. Chen, Biomaterials, 2009, 30, 79-88

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