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Isolation of At5PTase9 Mutants and Study of NaCl Tolerance in Arabidopsis

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This study focuses on isolating homozygous mutants of At5PTase9 and assessing their response to NaCl stress. Wild-type and mutant plants were evaluated for growth and ROS production under different conditions. Results provide insights into At5PTase9 function in salt stress response.

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Isolation of At5PTase9 Mutants and Study of NaCl Tolerance in Arabidopsis

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  1. 5PTase9 At2g01900 SALK_090899 SALK_090905 LP+RP LBb1+RP Isolation of homozygous mutants of At5ptase9 (Salk_090899) Salk_090899 Salk_090899 WT WT LP: TAT ACT TGC CAG CCC ATT TTG RP: TAC TGG TTT ATG CGT ATC CGG LBb1: ATT TTG CCG ATT TCG GAA C Supplemental Figure S1. Structure of At5PTase9 gene Upper panels: Molecular structure of the At5PTase9 gene, showing the positions of the T-DNA insertions. Bottom panel: verification of homozygosity of At5ptase9 mutants

  2. 150 mM NaCl WT (salk_090905) ½ MS ½ MS WT (salk_090905) Supplemental Figure S2. Growth of wild-type and At5ptase9 mutants in 150 mM NaCl. Wild-type and At5ptase9 mutants (Salk_090905) were germinated on 1/2 MS and after seven days transferred to plates supplemented with 150 mM NaCl or to 1/2 MS (control).

  3. sorbitol control 20 15 ROS production (Rel. units) 10 5 0 WT At5ptase9 9-OE Supplemental Figure S3. ROS production in wild-type and At5ptase9 mutants or overexpressors treated with 400 mM sorbitol

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