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AraC

AgaR ExuR NanR TdcA. GalR GatR GlpR. CdaR FucR SrlR. GntR MalT. NagC GalR. NagC GntR. ExuR. CdaR. IdnR TreR. LacI. GlpR RhaR. LldR MtlR. RbsR. AraC. DcuR. MalT.

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AraC

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  1. AgaR ExuR NanR TdcA GalR GatR GlpR CdaR FucR SrlR GntR MalT NagC GalR NagC GntR ExuR CdaR IdnR TreR LacI GlpR RhaR LldR MtlR RbsR AraC DcuR MalT Figure S1. Log2 expression ratio (y-axis) vs. locus tag (x-axis) plot showing catabolic regulons that are more highly expressed in E. coli MG1655* compared to E. coli MG1655 wild-type.

  2. Flh Fli Flg Figure S2. Log2 expression ratio (y-axis) vs. locus tag (x-axis) plot showing flagellar regulons that are more highly expressed in E. coli MG1655 wild-typecompared to E. coli MG1655*.

  3. Figure S3. Log2 expression ratio (y-axis) graph comparing average expression of selected regulons in E. coli MG1655* with E. coli MG1655 flhD.

  4. water cyc-dex dextrin glyc T-40 T-80 Nac-gal Nac-glu adonitl arabin arabitl cellob erythr fruct fuc gal gentib glc inositl lactose lactul malt mannitl mann melib met-glc psicose raffin rhamn sorbitl sucrose trehal turan xylitl pyr-met suc-met acetate aconit citrate formate galnt-lac galnt gnt gluc-N glunt aOH-but bOH-but gOH-but OH-phe itacon ket-but ket-glut ket-val lactate malon propion quinate sacchar sebac succin Br-succ succ-N glun-N ala-N D-ala L-ala ala-gly asn asp glu gly-asp gly-glu Figure S4. Catabolome analysis of E. coli MG1655* during growth on minimal glucose medium. Cells were grown to mid-log phase, harvested in chloramphenicol, loaded into BiOLOG GN2 Microplates, and measured every 15 minutes for 24 hours. The degree of color change in the well for the wild-type (green) is compared to the adapted strain (red). Yellow indicates a similar degree of carbon source oxidation. his OH-pro leu ornith phe pro pyroglu D-ser L-ser thr carnit N-butyr urocan inosine uridine thy phe-eth putresc N-eth but-diol glyc glyc-P gluc-P1 gluc-6P

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