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Effects of LXR Activation on Macrophage Polarization in Smokers and COPD Patients

This study investigates the impact of LXR activation on the polarization of alveolar macrophages from smoking controls and COPD patients. Alveolar macrophages were treated with or without the LXR agonist GW3965 at 1 µM and 10 µM for durations of 4, 24, and 48 hours. The expression levels of key macrophage markers, including HO-1, CD36, MR, and TLR4, were analyzed through PCR. Results indicate significant changes in mRNA expression levels, providing insights into the role of LXR in macrophage function in pulmonary disease contexts.

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Effects of LXR Activation on Macrophage Polarization in Smokers and COPD Patients

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  1. (A) (B) • Additional File 8 (C) (D) (E) (F) (G) (H) The effect of LXR activation on macrophage polarisation. Alveolar macrophages from smoking controls (A, C, E, and G) (n=8 apart from at 10 µM n=5) and COPD patients (B, D, F, and H) (n=9 apart from at 10 µM n=3) were treated with or without GW3965 (1 µM and 10 µM) for 4, 24 and 48 h. RNA was extracted for PCR analysis of HO-1 (A and B), CD36 (C and D), MR (E and F) and TLR4 (G and H) mRNA expression. Data shown are mean ± SEM of fold increase of mRNA expression above time matched controls.

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