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This study presents the mass spectra of compounds released from a single bead at different stages of peptide synthesis. The top spectrum depicts the starting peptide, while the middle and bottom spectra illustrate the results after the coupling of Fmoc-protected and azide-protected building blocks, respectively. The specific mass-to-charge ratios (m/z) of the molecules detected include [M+Na]+ and [M+H]+ forms, providing insight into the chemical composition and efficiency of the coupling reactions.
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641.29 [M+Na]+ 619.31 [M+H]+ 657.25 [M+K]+ 1437.39 [M+Na]+ 1453.39 [M+K]+ 1237.10 [M+Na]+ 1253.04 [M+K]+ Fig. 1 Mass spectra of compounds released from a single bead (top) starting peptide, (middle) after coupling of the Fmoc protected building block and (bottom) after coupling of the azide protected building block.