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生物感測器原理與應用

生物感測器原理與應用. Surface Plasmon Resonance Biosensors. Surface Plasmon Resonance. --- SPR is a real-time, label-free, optical detection method for studying the interaction of soluble analyte with immobilized ligand --- completely non-specific

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生物感測器原理與應用

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  1. 生物感測器原理與應用 Surface Plasmon Resonance Biosensors

  2. Surface Plasmon Resonance --- SPR is a real-time, label-free, optical detection method for studying the interaction of soluble analyte with immobilized ligand --- completely non-specific 1902 Wood – anomalous diffraction due to excitation of surface plasma waves 1968 Otto – the method of attenuated total reflection 1988 (1990) BIAcore

  3. Total Internal Reflectance n1 > n2 evanescent distance ± 100 nm

  4. Thin Conducting Film A medium of high refractive index A thin layer with good electric conductivity A medium of low refractive index

  5. Characteristics of SPW Gold is the most practical. (In) too expansive, (Na) violently reactive, (Cu, Al) too broad in their SPR response (Ag) too susceptible to oxidation

  6. Configuration of SPR

  7. BIO-SUPLAR 2

  8. BIAcore

  9. Real-Time Kinetics

  10. Sensorgram

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