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Water Source Influence on Algal Survivorship

Water Source Influence on Algal Survivorship. By Luke Beck Central Catholic HS PJAS 2010. Runoff. Part of the water cycle and describes the water that flows over a land surface. Surface runoff is runoff that occurs on land, typically creating or contributing to a ‘watershed.’

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Water Source Influence on Algal Survivorship

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  1. Water Source Influence on Algal Survivorship By Luke Beck Central Catholic HS PJAS 2010

  2. Runoff • Part of the water cycle and describes the water that flows over a land surface. • Surface runoff is runoff that occurs on land, typically creating or contributing to a ‘watershed.’ • Materials that are transported on surface runoff are fertilizers, petroleum, pesticides, herbicides, and salt. • Water source runoff pollution?

  3. Water Samples • Spring Water • Control group • Contains 7mg of sodium • Distilled Water • Purest water source • Water with all impurities removed • Tap Water • Purified to some extent • Contains 9mg of sodium

  4. Water Sample Continued • Creek Water • Suburban backyard creek • Flowing creek • Pond Water • Plant and aquatic life • Fertilizer drainage • River Water • Plenty of pollution • Flowing river

  5. Algal Importance • Base of aquatic food chain • Used as a bio-indicator for aquatic environments

  6. Euglena gracilis • Unicellular organism from the Protista Kingdom • Both autotrophic and heterotrophic • Found in ponds and puddles • Reproduce rapidly • Absorbs light readily

  7. Spectrophotometer • Device measuring light intensity or wavelengths of light • Used for measuring light absorption • Measures the light absorption of the algae • More absorption = more algae • Wavelength: 430nm

  8. Purpose • To determine the effects of common water sources on a natural water algal population, Euglena gracilis.

  9. Hypotheses • Null Hypothesis The water samples will have no significant effect on Euglena population growth. • Alternative Hypothesis The varying water samples will cause an effect on the algae growth.

  10. Materials • Science Kit Educator Spectrophotometer • Euglena gracilis Algae • Giant Eagle Spring Water • Giant Eagle Distilled Water • Tap Water • Creek Water • Pond Water • River Water • 13x100mm Borosilicate culture tubes • Pipettes • Pipette Pumps • Test Tube Racks • Window sill with sun exposure

  11. Procedure • Added the following ingredients to 13x100 mm culture tubes.

  12. Procedure • Spectrophotometer was used to take readings for 14 straight days. • Placed 13x100 mm culture tubes in racks and placed them on window sill.

  13. ANOVA Dunnett’s Test • Statistic test that compares variation within data groups to variation between means of the groups. • If ANOVA reveals statistical significance, each variable group can be compared to the control to examine significance of each variable.

  14. ANOVA Results 4mL Groups

  15. 4mL Water Effects on Euglena P-value=1.55E-28 Absorbance Days

  16. Dunnett’s Test T-crit = 3.62 T-crit < T value = Significant

  17. ANOVA Results 2mL Groups

  18. 2mL Concentration Averages P-value=5.59E-34 Absorbance Days

  19. Dunnett’s Test T-crit = 3.62

  20. Conclusion • The null hypothesis that the water sources will cause variation in algal growth was accepted in the 4mL of tap water, 4mL of river water, and 2mL of the creek water. • The null hypothesis was rejected in both distilled water tests, the 2mL tap water, 4mL creek water, both pond water tests, and the 2mL of river water.

  21. Limits and Extensions • Limits • Only one type of algae was used in this project. • The cultures of algae are not sterile (non-monocultural), thus it is possible for other species to be present. • Varying light for each day. • Extensions • Check ph of the waters. • Grow algae at different temperatures. • Confirm cell population counts with hemacytometer.

  22. References • http://www.fcps.edu/islandcreekes/ecology/euglena.htm • http://www.ruf.rice.edu/~bioslabs/methods/protein/spectrophotometer.html • http://my.gianteagle.com/health/healthnotes.aspx?org=ge%2Cge%2Cge&ContentID=1992003 • http://ww2010.atmos.uiuc.edu/(Gh)/guides/mtr/hyd/run.rxml

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