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Statistics

Statistics. Introduction 1.) All measurements contain random error results always have some uncertainty 2.) Uncertainty are used to determine if two or more experimental results are equivalent or different Statistics is used to accomplish this task.

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Statistics

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  1. Statistics • Introduction • 1.)All measurements contain random error • results always have some uncertainty 2.) Uncertainty are used to determine if two or more experimental results are equivalent or different • Statistics is used to accomplish this task Is the mutant (transgenic) mouse significantly fatter than the normal (wild-type) mouse? Statistical Methods Provide Unbiased Means to Answer Such Questions. Masuzaki, H., et. al Science (2001), 294(5549), 2166

  2. Statistics • Gaussian Curve • 1.)For a series of experimental results with only random error: (i) A large number of experiments done under identical conditions will yield a distribution of results. (ii) Distribution of results is described by a Gaussian or Normal Error Curve High population about correct value low population far from correct value Number of Occurrences Value

  3. Statistics • Gaussian Curve • 2.)Any set of data (and corresponding Gaussian curve) can be characterized by two parameters: (i) Mean or Average Value ( ) where: n = number of data points xi = value of data point number i = value1 + value2 + value3… valuen (ii)Standard Deviation (s) Smaller the standard deviation is, more precise the measurement is.

  4. Statistics • Gaussian Curve • 3.)Other Terms Used to Describe a Data Set (i) Variance: Related to the standard deviation • Used to describe how “wide” or precise a distribution of results is variance = (s)2 where: s = standard deviation (ii)Range: difference in the highest and lowest values in a set of data • Example: From the 4 light bulb measurements High Value = 855 hours Low Value = 783 hours Range = High Value – Low Value = 855 – 783 = 72 hours

  5. Statistics • Gaussian Curve • 3.)Other Terms Used to Describe a Data Set (iii) Median: The value in a set of data which has an equal number of data values above it and below it • For odd number of data points, the median is actually the middle value • For even number of data points, the median is the value halfway between the two middle values • Example: Data Set: 1.19, 1.23, 1.25, 1.45 ,1.51 mean( ) = 1.33 Data Set: 1.19, 1.23, 1.25, 1.45 mean( ) = 1.28 median = 1.24 Median value Median value

  6. Statistics • Gaussian Curve (iii)Example: For the following bowling scores 116.0, 97.9, 114.2, 106.8 and 108.3, find the mean, median, range and standard deviation.

  7. Statistics • Gaussian Curve • 4.)Relating Terms Back to the Gaussian Curve (i) Formula for a Gaussian curve wheree = base of natural logarithm (2.71828…) m≈ (mean) s ≈ s (standard deviation) mean Entire area under curve is normalized to one ± standard deviation

  8. Statistics • Standard Deviation and Probability • 1.)By knowing the standard deviation (s) and the mean ( ) of a set of result (and the corresponding Gaussian curve) (i) The probability of the next result falling in any given range can be calculated by: (ii) The probability of a result falling in that portion of the Gaussian curve is equal to the normalized area of the curve in that portion. (iii) Example: 68.3% of the area of a Gaussian curve occurs between the values -1s and +1s ( ± 1s) Thus, any new result has a 68.3% chance of falling within this range. Probability of Measuring a value in a certain range is equal to the area of that range

  9. Statistics Standard Deviation and Probability • - Area under curve from mean value and result. • Total ½ area is 0.5. • Remaining area is 0.5 – Area. • Example: • z = 1.3area from mean to 1.3 is 0.403 •  area from infinity to 1.3 is 0.5 – 0.403 = 0.097

  10. Statistics • Standard Deviation and Probability (iii) Example: A bowler has a mean score of 108.6 and a standard deviation of 7.1. What fraction of the bowler’s scores will be less than 80.2?

  11. Statistics • Standard Deviation and Probability • 2.)Knowing the standard deviation (s) of a data set indicates the precision of a measurement (i) Common intervals used for expressing analytical results are shown below: (ii) The precision of many analytical measurements is expressed as: • There is only a ~5% chance (1 out of 20) that any given measurement on the sample will be outside of this range

  12. Statistics • Standard Deviation and Probability • 4.)The precision of a mean (average) result is expressed using a confidence interval (i) Relationship between the true mean value (m) and the measured mean ( ) is given by: where:s = standard deviation n = number of measurements t = student’s t value degrees of freedom = (n-1) Confidence interval Note: As n increases, the confidence interval becomes smaller (m becomes more precisely known)

  13. Statistics • Standard Deviation and Probability • 4.)The precision of a mean (average) result is expressed using a confidence interval (ii) Student’s t • Statistical tool frequently used to express confidence intervals From number of measurements (n-1) A probability distribution that addresses the problem of estimating the mean of a normally distributed population when the sample size is small. Population standard deviation (s) is unknown and has to estimated from the data using s.

  14. Statistics • Standard Deviation and Probability • 4.)The precision of a mean (average) result is expressed using a confidence interval (iii) The meaning of Confidence Interval • The determine the “true” mean need to collect an infinite number of data points. - obviously not possible • Confidence interval tells us the probability that the range of numbers contains the “true” mean. 50% confidence interval  range of numbers only contains true mean 50% of the time 90% confidence interval  range of numbers contains true mean 90% of the time. “true” mean 50% of data sets do not contain true mean

  15. Statistics • Standard Deviation and Probability (iii) Example: For the following bowling scores 116.0, 97.9, 114.2, 106.8 and 108.3, a bowler has a mean score of 108.6 and a standard deviation of 7.1. What is the 90% confidence interval for the mean?

  16. Statistics • Standard Deviation and Probability • 5.)Comparison of Two Data Sets (i) To determine if two results obtained by the same method are statistically the same, use the following formula to determine a calculated t: where: = mean results of samples 1 & 2 n1, n2 = number of measurements of samples 1 & 2 spooled = “pooled” standard deviation Requires standard deviation from the two data sets be similar.

  17. Statistics • Standard Deviation and Probability • 5.)Comparison of Two Data Sets (ii) Compare calculated t to the corresponding value in the Student’s t probability table. • Use the desired %confidence level at the appropriate Degrees of freedom • Degrees of Freedom = (n1 + n2 -2) (iii) If calculated t is greater than the value in the Student’s t probability table, then the two results are significantly different at the given % confidence level. • Easier to achieve for lower %confidence level Calculated t needs to be less than table value

  18. Statistics • Standard Deviation and Probability • 5.)Comparison of Two Data Sets (iv) Example: The amount of 14CO2 in a plant sample is measured to be: 28, 32, 27, 39 & 40 counts/min (mean = 33.2). The amount of radioactivity in a blank is found to be: 28, 21, 28, & 20 counts/min (mean = 24.2). Are the mean values significantly different at a 95% confidence level?

  19. Statistics • Standard Deviation and Probability • 5.)Comparison of Two Data Sets (iv) Example: Degrees of Freedom = (5 + 4 – 2) = 7 From Student’s t probability table: Degrees of Freedom (7) 95% Confidence level Calculated t (2.48) > 2.365 The results are significantly different at a 95% confidence level, but not at 98% or higher confidence levels

  20. Statistics • Standard Deviation and Probability • 6.)Comparison of Two Methods (i) To determine if the results of two methods for the same sample are the same, use the following formula to determine a calculated t: where: = difference in the mean values of the two methods n = number of samples analyzed by each method sd = (ii) Degree of Freedom = (n - 1) (iii) If calculated t is greater than the value in the Student’s t probability table, then the two methods are significantly different at the given % confidence level.

  21. Statistics • Standard Deviation and Probability • 6.)Comparison of Two Methods (iv) Example: Two methods for measuring cholesterol in blood provide the following results: Are these methods significantly different at the 95% confidence level?

  22. Statistics • Standard Deviation and Probability • 6.)Comparison of Two Methods (iv) Example: 95% Confidence level Degrees of Freedom (6-1 =5) Calculated t (1.20) ≤ 2.571 The results are not significantly different at a 95% confidence level.

  23. Statistics • Dealing with Bad Data • 1.)Q Test (i) Method used to decide whether or not to reject a “bad” data point. (ii) Procedure: • Arrange Data in order of increasing value. • Determine the lowest and highest values an the total range of values. Example: 12.47 12.48 12.53 12.56 12.67 • Determine the difference between the “bad” data point and the nearest value. - Calculate the “Q value” Questionable point Range = 0.20 gap = 0.11

  24. Statistics • Dealing with Bad Data • 1.)Q Test (ii) Procedure: 4. Compare the calculated Q value to those in Tables at the same value of n and the desired %confidence level. - n: total number of values or observations - For example, at n = 5 and 90% confidence, the value of Q is 0.64 - Since: Q (calculated) ≤ Q (table) 0.55 ≤ 0.64 - data point 12.67 can not be rejected at the 90% confidence level (iii) Although the Q-test is valuable in eliminating bad data, common sense and repeating experiments with questionable results are usually more helpful.

  25. Statistics • Dealing with Bad Data • 1.)Q Test (ii) Example: For the following bowling scores 116.0, 97.9, 114.2, 106.8 and 108.3, a bowler has a mean score of 108.6 and a standard deviation of 7.1. Using the Q test, decide whether the number 97.9 should be discarded.

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