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The Effect of Beta Carotene on Plants Infected with Agrobacterium Tumefaciens

The Effect of Beta Carotene on Plants Infected with Agrobacterium Tumefaciens. Christina Adams Grade 9. Problem. Will Beta Carotene have any role in preventing the effect of Agrobacterium tumefaciens on violets ?

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The Effect of Beta Carotene on Plants Infected with Agrobacterium Tumefaciens

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  1. The Effect of Beta Carotene on Plants Infected with AgrobacteriumTumefaciens Christina Adams Grade 9

  2. Problem Will Beta Carotene have any role in preventing the effect of Agrobacterium tumefaciens on violets? I picked this experiment because, over the summer, I went to Peterhof in Russia and was inspired by the beauty of the gardens there. Botany and the science of plants was interesting to me from then on.

  3. Research Beta Carotene is a red-orange pigment found in various types of plants. Beta Carotene is converted in the body to Vitamin A. Agrobacteriumtumefaciens is the bacteria that causes tumor formation in over 140 different dicot (plants with 2 or more embyrotic leaves) plants. A. tumefacienscan live freely in soil or inside plants as a parasite

  4. Hypothesis If Beta Carotene is used, then it should have no effect on preventing damage from the Agrobacteriumtumefaciens.

  5. Materials 24 violets Beta Carotene solution Disinfectant Bunsen Burner Inoculating Needle AgrobacteriumTumefaciens

  6. Procedure 8 plants were placed in each pot Pot A is inoculated with A. tumefaciensand watered with 100ml of beta carotene twice weekly. Pot B is inoculated with A. tumefaciensand watered with 100ml of distilled water twice weekly. Pot C is not inoculated, but watered with 100ml of distilled water twice weekly. The height of the plants will be documented over a 30-day period.

  7. Data

  8. Data

  9. Data

  10. Conclusion • If Beta Carotene is used then it will have no effect on A. tumefaciensinfections. • This was not supported. • The control group had the highest growth rate followed by the beta carotene group. • The bacterial group had the lowest growth rate Growth stopped after two weeks and plants began to brown

  11. Conclusion (cont) How to improve experiment: - use different types of plants, such as monocot plants, and different types of solution to test the the effectiveness of prevention

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