1 / 12

SCMD: Saccharomyces Cerevisiae Morphological Database

SCMD is a collection of micrographs of budding yeast mutants analyzed using the CalMorph program to study characteristics like cell wall, nucleus location, and actin distribution. Explore 84,407 processed micrographs of 4,495 mutants.

fsusan
Télécharger la présentation

SCMD: Saccharomyces Cerevisiae Morphological Database

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. SCMD:Saccharomyces Cerevisiae Morphological Database Taro L. Saito1,2, Miwaka Ohtani1,2, Hiroshi Sawai2, Fumi Sano2,4, Ayaka Saka2,4, Daisuke Watanabe2,4, Masashi Yukawa2,4, Yoshikazu Ohya2,4 and Shinichi Morishita*,2,3 • Department of Computer Science, Graduate School of Information Science and Technology, University of Tokyo • ２． Institute for Bioinformatics and Research and Development, Japan Science and Technology Corporation • ３. Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo • ４． Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo

  2. What is SCMD ? • SCMD is a collection of micrographs of budding yeast mutants. • Our image processing program, called CalMorph, clips individual cells from micrographs, then analyzes the characteristics of them. • Currently following number of yeast mutants and their micrographs are analyzed. • Mutants analyzed: 4,495 • Micrographs Processed: 84,407 (x 3) • Cells retrieved: 1,643,835

  3. Micrographs • Three type of stains are used to see • Cell wall • FITC-ConA • Nucleus location • DAPI • Actin distribution • Rh-ph • All micrographs can be seen in the viewer provided in SCMD Cell wall Nucleus Actin image processing

  4. stain FITC-ConA DAPI Rh-ph

  5. Cell Parameters

  6. Categorizing by Bud Size • View of ‘group by bud size’ no bud small medium large yor202w his3

  7. Nucleus Location Categories • We categorized the states of nucleus locations into 7 groups. • State A, A1, B, C are regular status. • State D, E, F seldom occur.

  8. Categorizing by Nucleus Location • View of ‘group by nucleus location’ A A1 B C ybl052c sas3

  9. Actin Localization Categories

  10. Categorizing by Actin Localization • View of ‘group by actin localization’ A B iso api E F

  11. Morphology Search Interface

  12. Searching Similar Shape Mutants input shape query result

More Related