ABSTRACT

1. INCDTIM (b) (a) (c) (d) THE NON-INVASIVE ASSESSMENT OF THE EFFICACY OF GOLD NANOPARTICLES-ANTHOCYANINS NANOMATERIALS ON CUTANEOUS PATHOLOGY Liliana Olenic1,Adriana Vulcu1, Camelia Grosan1, Luminita David2, Bianca Moldovan2, Maria Perde-Schrepler3, Adriana Gabriela Filip4 , Maria Crisan4, Ioana Chiorean5 1National Institute for Research and Development of Isotopic and Molecular Technologies, 65-103 Donath Street, Cluj-Napoca, Romania 2Faculty of Chemistry and Chemical Engineering, 11 Arany Janos Street RO 400028, Cluj-Napoca, Romania 3“Ion Chiricuţă” Oncology Institute, 34-36 Republicii Street Cluj-Napoca, Romania 4Faculty of General Medicine, Iuliu Haţieganu University of Medicine and Pharmacy Strada Emil Isac 13, Cluj-Napoca, Romania 5Faculty of Mathematics and Informatics, , Babeş-Bolyai –University, Kogălniceanu Street, 1, Cluj-Napoca, Romania 400084 • ABSTRACT • Fruits of Romanian native plants from Adoxaceae family (European Cranberrybush - Viburnum opulusL. –E1 and European Black Elderberry - Sambucusnigra L.-E2) have biological activity mainly due to their high content of anthocyanins and other polyphenols. They were used to obtain new nanomaterials based on gold nanoparticles. We have controled the size, shape and surface morphology of these nanomaterials(AuNPs-E1, AuNPs-E2) prepared by using a green method; the new nanomaterials have been used in medicine to treat some skin diseases. • We have evaluated their in vitro and in vivo effect and we have identified the effectiveness of new topic therapies based on nanotechnology using high frequency ultrasound. EXPERIMENTAL Figure 1. (a) Viburnum opulus L., berries; (b) Sambucus Nigra L., berries;(c) And (d) TEM images of AuNPs with E1 respectively E2 Figure 2. UV-Vis spectra of (a) AuNPs-E1; (b) AuNPs-E2 RESULTS AND DISCUSSIONS 2. In vivo toxicity studies 3. Evaluation of new prepared nanomaterials on skin diseases 1. In vitro toxicity studies Figure 5. Level of malondialdehyde for different lots Figure 8. Images token with high-frequency ultrasound before and after topical application of new nanomaterials for 2 weeks Figure 3. The viability of HaCaTcell and A431 cells function of nanomaterials concentration Figure 6.Glutatione level for different lots Figure 9. The histogram of skin thickness for 8 patients before and after treatment with nanomaterials Figure 4. Compared of cell survival between the two line of cells with the two types of nanoparticles Figure 7. Edema of foot after 2 h treatment • CONCLUSION • Gold nanoparticles functionalized with anthocyanins extracted from Viburnum opulus L. and Sambucus nigra L. (AuNPs-E1 and AuNPs-E2) were prepared and characterized by UV-Vis and FTIR spectroscopy and by Transmission Electron Spectroscopy (TEM) (diameters between 25-100 nm) • There were assessed cytotoxicity tests of the extract alone and of the functionalized nanoparticles; • To study the antiinflammatory effect, keratinocytes treated with non-toxic doses of anthocyanins and nanoparticles were exposed to increasing doses of UVB irradiation • The histograms of the skin thickness for 8 patients presented an involution of diseases after treatment, fact that indicates the very good antiinflammatory effect of our nanomaterials. Acknowledgement This work was elaborated in the frame project no 147/2012, through the program “Partenerships in priority areas-PN II”, developed with the support of ANCS, CNDI-UEFISCDI