1 / 19

Sordaria Lab

Sordaria Lab. Lab 3: Mitosis & Meiosis. Exercise 3B Studies crossing over and recombination during meiosis using asci from crosses between wild and mutants spores in the fungus Sordaria fimicola . Arrangements will be used to estimate the percentage of crossing over that occurs.

gent
Télécharger la présentation

Sordaria Lab

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Sordaria Lab

  2. Lab 3: Mitosis & Meiosis Exercise 3B • Studies crossing over and recombination during meiosis using asci from crosses between wild and mutants spores in the fungus Sordaria fimicola. • Arrangements will be used to estimate the percentage of crossing over that occurs.

  3. Lab Objectives You should be able to: • Calculate the map distance of a particular gene from a chromosome's center for between two genes using an organism of your choice in a controlled experiment • Demonstrate the role of meiosis in the formation of gametes using an organism of your choice, in a controlled experiment

  4. Class: Ascomycetes (Cup Fungi) • Largest division of fungi, terrestrial • All produce spores in pouch-like asci

  5. % Crossing over

  6. Non-Crossing over

  7. Crossing over

  8. Innoculating the Plate

  9. Counting the Asci • Always 8 spores in each Asci (also called conidia) • Used in genetic recombination studies

  10. # showing crossing-over?

  11. # showing crossing-over?

  12. # showing crossing-over?

  13. # showing crossing-over?

  14. # showing crossing-over?

  15. # showing crossing-over?

  16. # showing crossing-over?

  17. # showing crossing-over?

  18. # showing crossing-over?

  19. Calculate the Map Units • Calculate the percent of crossovers by dividing the number of crossover asci (2:2:2:2 or 2:4:2) by the total number of asci x 100. • Calculate the map distance, divide the percent of crossover asci by 2. • % of crossover asci is divided by 2 because only half of the spores in each ascus are the result of crossing over.

More Related