1 / 34

L. II-3.

L. II-3. S. 14-12. L. 13-9. L. 13-3,4. L. 13-5. In situ study of energy metabolism: Monitoring of high-energy phosphorus metabolite levels in perfused rat hearts using 31 P NMR spectroscopy. CrP. PCr. -ATP. -ATP. -ATP. Reperfusion in the presence of cardioprotective agents.

geoff
Télécharger la présentation

L. II-3.

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. L. II-3. S. 14-12.

  2. L. 13-9

  3. L. 13-3,4

  4. L. 13-5

  5. In situ study of energy metabolism:Monitoring of high-energy phosphorus metabolite levels in perfused rat hearts using 31P NMR spectroscopy CrP PCr -ATP -ATP -ATP Reperfusion in the presenceof cardioprotective agents Reperfusion Pi Ischaemia ATP ATP ATP Normoxia

  6. Effect of PARP inhibitors on the recovery of creatine phosphate during ischemia-reperfusion

  7. Effect of PARP inhibitors on the recovery of ATP during ischemia-reperfusion

  8. Effect of PARP inhibitors on the utilization of inorganic phosphate during ischemia-reperfusion

  9. L. 13-6,7

  10. L. 13-8,12

  11. L. 13-10,11

  12. Electron transfer as a form of energy transfer Electron affinity, oxidative/reductíve capacity electromotive forceE = E° +0.026V/n ln[ox]/[red] G = -nF E

  13. Deficiency: pellagra (dermatitis, diarrhea, dementia, death)

  14. Regulation of metabolic pathways • controlling the amounts of enzymes • controlling the catalytic activities of enzymes • controlling the accessibilityof substrates

  15. Possible fates of glucose

  16. Glycolysis • glykys= sweet, lysis=splitting; degradation of glucose • the process of the largest carbon flux in most cells • sole energy source for many kinds of cells (e.g. erythrocytes, brain, renal medulla, sperm) • anaerobic microorganisms are entirely dependent on glycolysis (e.g. Clostridium tetani - tetanus (lockjaw)Clostridium botulinum - botulismBacterides fragilis - various types of infection) • the enzymes involved are almost completely conserved from yeast to human

  17. All intermediates of glycolysis are phosphorylated • they cannot leave the cell(negative charge, lack of transporters; concentration gradient) • energy is conserved(phosphorylation potential of ATP is not lost completely) • enzymes are phosphorylated at their active sites(phosphorylation  rearrangement  decrease of the activation barrier AND increase of specificity) • Mg2+ is necessary for each steps

  18. Energy balance of glycolysis Glc + 2 NAD+ + 2 ADP + 2 Pi 2 Pyr + 2 NADH + 2 H+ + 2 ATP + 2 H2OG’° = -85 kJ/mol Glc + 2 NAD+  2 Pyr + 2 NADH + 2 H+ G’° = -146 kJ/mol and it is only a small fraction of total available energy of the glucose molecule! (see TCA cycle later) 2 ADP + 2 Pi 2 ATP + 2 H2O G’° = 61 kJ/mol

  19. “Essentially irreversible” reactions in glycolysis

  20. Anabolic reactions

More Related